Whole-genome sequencing of bloodstream Staphylococcus aureus isolates does not distinguish bacteraemia from endocarditis

Berit Lilje, Rasmus Vedby Rasmussen, Anders Dahl, Marc Stegger, Robert Leo Skov, Vance G Fowler, Kim Lee Ng, Kristoffer Kiil, Anders Rhod Larsen, Andreas Petersen, Helle Krogh Johansen, Henrik Carl Schønheyder, Magnus Arpi, Flemming S Rosenvinge, Eva Korup, Ulla Høst, Christian Hassager, Sabine Ute Alice Gill, Thomas Fritz Hansen, Thor Bech JohannesenJesper Smit, Peter Søgaard, Paal Skytt Andersen, Niels Eske-Bruun

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Abstract

Most Staphylococcus aureus isolates can cause invasive disease given the right circumstances, but it is unknown if some isolates are more likely to cause severe infections than others. S. aureus bloodstream isolates from 120 patients with definite infective endocarditis and 121 with S. aureus bacteraemia without infective endocarditis underwent whole-genome sequencing. Genome-wide association analysis was performed using a variety of bioinformatics approaches including SNP analysis, accessory genome analysis and k-mer based analysis. Core and accessory genome analyses found no association with either of the two clinical groups. In this study, the genome sequences of S. aureus bloodstream isolates did not discriminate between bacteraemia and infective endocarditis. Based on our study and the current literature, it is not convincing that a specific S. aureus genotype is clearly associated to infective endocarditis in patients with S. aureus bacteraemia.

Original languageEnglish
JournalMicrobial Genomics
Volume3
Issue number11
Number of pages11
ISSN2057-5858
DOIs
Publication statusPublished - 2017

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Genome-Wide Association Study
Computational Biology
Single Nucleotide Polymorphism

Keywords

  • Journal Article

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Lilje, B., Rasmussen, R. V., Dahl, A., Stegger, M., Skov, R. L., Fowler, V. G., ... Eske-Bruun, N. (2017). Whole-genome sequencing of bloodstream Staphylococcus aureus isolates does not distinguish bacteraemia from endocarditis. Microbial Genomics, 3(11). https://doi.org/10.1099/mgen.0.000138
Lilje, Berit ; Rasmussen, Rasmus Vedby ; Dahl, Anders ; Stegger, Marc ; Skov, Robert Leo ; Fowler, Vance G ; Ng, Kim Lee ; Kiil, Kristoffer ; Larsen, Anders Rhod ; Petersen, Andreas ; Johansen, Helle Krogh ; Schønheyder, Henrik Carl ; Arpi, Magnus ; Rosenvinge, Flemming S ; Korup, Eva ; Høst, Ulla ; Hassager, Christian ; Gill, Sabine Ute Alice ; Hansen, Thomas Fritz ; Johannesen, Thor Bech ; Smit, Jesper ; Søgaard, Peter ; Skytt Andersen, Paal ; Eske-Bruun, Niels. / Whole-genome sequencing of bloodstream Staphylococcus aureus isolates does not distinguish bacteraemia from endocarditis. In: Microbial Genomics. 2017 ; Vol. 3, No. 11.
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title = "Whole-genome sequencing of bloodstream Staphylococcus aureus isolates does not distinguish bacteraemia from endocarditis",
abstract = "Most Staphylococcus aureus isolates can cause invasive disease given the right circumstances, but it is unknown if some isolates are more likely to cause severe infections than others. S. aureus bloodstream isolates from 120 patients with definite infective endocarditis and 121 with S. aureus bacteraemia without infective endocarditis underwent whole-genome sequencing. Genome-wide association analysis was performed using a variety of bioinformatics approaches including SNP analysis, accessory genome analysis and k-mer based analysis. Core and accessory genome analyses found no association with either of the two clinical groups. In this study, the genome sequences of S. aureus bloodstream isolates did not discriminate between bacteraemia and infective endocarditis. Based on our study and the current literature, it is not convincing that a specific S. aureus genotype is clearly associated to infective endocarditis in patients with S. aureus bacteraemia.",
keywords = "Journal Article",
author = "Berit Lilje and Rasmussen, {Rasmus Vedby} and Anders Dahl and Marc Stegger and Skov, {Robert Leo} and Fowler, {Vance G} and Ng, {Kim Lee} and Kristoffer Kiil and Larsen, {Anders Rhod} and Andreas Petersen and Johansen, {Helle Krogh} and Sch{\o}nheyder, {Henrik Carl} and Magnus Arpi and Rosenvinge, {Flemming S} and Eva Korup and Ulla H{\o}st and Christian Hassager and Gill, {Sabine Ute Alice} and Hansen, {Thomas Fritz} and Johannesen, {Thor Bech} and Jesper Smit and Peter S{\o}gaard and {Skytt Andersen}, Paal and Niels Eske-Bruun",
year = "2017",
doi = "10.1099/mgen.0.000138",
language = "English",
volume = "3",
journal = "Microbial Genomics",
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Lilje, B, Rasmussen, RV, Dahl, A, Stegger, M, Skov, RL, Fowler, VG, Ng, KL, Kiil, K, Larsen, AR, Petersen, A, Johansen, HK, Schønheyder, HC, Arpi, M, Rosenvinge, FS, Korup, E, Høst, U, Hassager, C, Gill, SUA, Hansen, TF, Johannesen, TB, Smit, J, Søgaard, P, Skytt Andersen, P & Eske-Bruun, N 2017, 'Whole-genome sequencing of bloodstream Staphylococcus aureus isolates does not distinguish bacteraemia from endocarditis', Microbial Genomics, vol. 3, no. 11. https://doi.org/10.1099/mgen.0.000138

Whole-genome sequencing of bloodstream Staphylococcus aureus isolates does not distinguish bacteraemia from endocarditis. / Lilje, Berit; Rasmussen, Rasmus Vedby; Dahl, Anders; Stegger, Marc; Skov, Robert Leo; Fowler, Vance G; Ng, Kim Lee; Kiil, Kristoffer; Larsen, Anders Rhod; Petersen, Andreas; Johansen, Helle Krogh; Schønheyder, Henrik Carl; Arpi, Magnus; Rosenvinge, Flemming S; Korup, Eva; Høst, Ulla; Hassager, Christian; Gill, Sabine Ute Alice; Hansen, Thomas Fritz; Johannesen, Thor Bech; Smit, Jesper; Søgaard, Peter; Skytt Andersen, Paal; Eske-Bruun, Niels.

In: Microbial Genomics, Vol. 3, No. 11, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Whole-genome sequencing of bloodstream Staphylococcus aureus isolates does not distinguish bacteraemia from endocarditis

AU - Lilje, Berit

AU - Rasmussen, Rasmus Vedby

AU - Dahl, Anders

AU - Stegger, Marc

AU - Skov, Robert Leo

AU - Fowler, Vance G

AU - Ng, Kim Lee

AU - Kiil, Kristoffer

AU - Larsen, Anders Rhod

AU - Petersen, Andreas

AU - Johansen, Helle Krogh

AU - Schønheyder, Henrik Carl

AU - Arpi, Magnus

AU - Rosenvinge, Flemming S

AU - Korup, Eva

AU - Høst, Ulla

AU - Hassager, Christian

AU - Gill, Sabine Ute Alice

AU - Hansen, Thomas Fritz

AU - Johannesen, Thor Bech

AU - Smit, Jesper

AU - Søgaard, Peter

AU - Skytt Andersen, Paal

AU - Eske-Bruun, Niels

PY - 2017

Y1 - 2017

N2 - Most Staphylococcus aureus isolates can cause invasive disease given the right circumstances, but it is unknown if some isolates are more likely to cause severe infections than others. S. aureus bloodstream isolates from 120 patients with definite infective endocarditis and 121 with S. aureus bacteraemia without infective endocarditis underwent whole-genome sequencing. Genome-wide association analysis was performed using a variety of bioinformatics approaches including SNP analysis, accessory genome analysis and k-mer based analysis. Core and accessory genome analyses found no association with either of the two clinical groups. In this study, the genome sequences of S. aureus bloodstream isolates did not discriminate between bacteraemia and infective endocarditis. Based on our study and the current literature, it is not convincing that a specific S. aureus genotype is clearly associated to infective endocarditis in patients with S. aureus bacteraemia.

AB - Most Staphylococcus aureus isolates can cause invasive disease given the right circumstances, but it is unknown if some isolates are more likely to cause severe infections than others. S. aureus bloodstream isolates from 120 patients with definite infective endocarditis and 121 with S. aureus bacteraemia without infective endocarditis underwent whole-genome sequencing. Genome-wide association analysis was performed using a variety of bioinformatics approaches including SNP analysis, accessory genome analysis and k-mer based analysis. Core and accessory genome analyses found no association with either of the two clinical groups. In this study, the genome sequences of S. aureus bloodstream isolates did not discriminate between bacteraemia and infective endocarditis. Based on our study and the current literature, it is not convincing that a specific S. aureus genotype is clearly associated to infective endocarditis in patients with S. aureus bacteraemia.

KW - Journal Article

U2 - 10.1099/mgen.0.000138

DO - 10.1099/mgen.0.000138

M3 - Journal article

C2 - 29208121

VL - 3

JO - Microbial Genomics

JF - Microbial Genomics

SN - 2057-5858

IS - 11

ER -