Whole exome sequencing of 28 families of Danish descent reveals novel candidate genes and pathways in developmental dysplasia of the hip

Maja Dembic*, Lars van Brakel Andersen, Martin Jakob Larsen, Inger Mechlenburg, Kjeld Søballe, Jens Michael Hertz

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Developmental dysplasia of the hip (DDH) is a common condition involving instability of the hip with multifactorial etiology. Early diagnosis and treatment are critical as undetected DDH is an important cause of long-term hip complications. Better diagnostics may be achieved through genetic methods, especially for patients with positive family history. Several candidate genes have been reported but the exact molecular etiology of the disease is yet unknown. In the present study, we performed whole exome sequencing of DDH patients from 28 families with at least two affected first-degree relatives. Four genes previously not associated with DDH (METTL21B, DIS3L2, PPP6R2, and TM4SF19) were identified with the same variants shared among affected family members, in more than two families. Among known association genes, we found damaging variants in DACH1, MYH10, NOTCH2, TBX4, EVC2, OTOG, and SHC3. Mutational burden analysis across the families identified 322 candidate genes, and enriched pathways include the extracellular matrix, cytoskeleton, ion-binding, and detection of mechanical stimulus. Taken altogether, our data suggest a polygenic mode of inheritance for DDH, and we propose that an impaired transduction of the mechanical stimulus is involved in the etiopathological mechanism. Our findings refine our current understanding of candidate causal genes in DDH, and provide a foundation for downstream functional studies.

Original languageEnglish
JournalMolecular Genetics and Genomics
Volume298
Issue number2
Pages (from-to)329-342
ISSN1617-4615
DOIs
Publication statusPublished - Mar 2023

Keywords

  • DDH
  • Detection of mechanical stimulus
  • DNA mutation
  • Whole exome sequencing
  • Exome Sequencing
  • Pedigree
  • Humans
  • Developmental Dysplasia of the Hip
  • Denmark
  • Hip Dislocation, Congenital/genetics

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