Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA

Agnieszka Sałamaszyńska-Guz*, Izabela Serafińska, Paweł Bącal, Stephen Douthwaite

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

28 Downloads (Pure)

Abstract

Campylobacter jejuni is a bacterial pathogen that is generally acquired as a zoonotic infection from poultry and animals. Adhesion of C. jejuni to human colorectal epithelial cells is weakened after loss of its cj0588 gene. The Cj0588 protein belongs to the type I group of TlyA (TlyAI) enzymes, which 2′-O-methylate nucleotide C1920 in 23S rRNA. Slightly longer TlyAII versions of the methyltransferase are found in actinobacterial species including Mycobacterium tuberculosis, and methylate not only C1920 but also nucleotide C1409 in 16S rRNA. Loss of TlyA function attenuates virulence of both M. tuberculosis and C. jejuni. We show here that the traits impaired in C. jejuni null strains can be rescued by complementation not only with the original cj0588 (tlyAI) but also with a mycobacterial tlyAII gene. There are, however, significant differences in the recombinant phenotypes. While cj0588 restores motility, biofilm formation, adhesion to and invasion of human epithelial cells and stimulation of IL-8 production in a C. jejuni null strain, several of these properties are further enhanced by the mycobacterial tlyAII gene, in some cases to twice the original wild-type level. These findings strongly suggest that subtle changes in rRNA modification patterns can affect protein synthesis in a manner that has serious consequences for bacterial pathogenicity.

Original languageEnglish
Article numbere13199
JournalCellular Microbiology
Volume22
Issue number7
Number of pages10
ISSN1462-5814
DOIs
Publication statusPublished - Jul 2020

Keywords

  • bacterial motility
  • biofilms
  • capreomycin resistance
  • epithelial cell invasion
  • rRNA 2′-O-methylation

Fingerprint

Dive into the research topics of 'Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA'. Together they form a unique fingerprint.

Cite this