TY - JOUR
T1 - Urinary plasmin activates collecting duct ENaC current in preeclampsia
AU - Buhl, Kristian B
AU - Friis, Ulla G
AU - Svenningsen, Per
AU - Gulaveerasingam, Ambika
AU - Ovesen, Per Glud
AU - Frederiksen-Møller, Britta
AU - Jespersen, Bente
AU - Bistrup, Claus
AU - Jensen, Boye L
PY - 2012
Y1 - 2012
N2 - In nephrotic syndrome, plasminogen is aberrantly filtered from plasma to the urinary space and activated along the tubular system. In vitro, plasmin increases ENaC current by proteolytic cleavage of the γ-subunit. It was hypothesized that preeclampsia is associated with plasmin-dependent ability of tubular fluid to activate ENaC. Urine was sampled from 16 preeclamptic (PE) patients and 17 normotensive pregnant women (Ctrl). Urine was analyzed for plasmin(ogen), creatinine, albumin, aldosterone, Na(+), K(+), proteolytic activity, and for its effect on inward current in cortical collecting duct cells (M1 cells) by whole-cell patch clamp. In PE, urine plasmin(ogen): creatinine ratio was elevated 40-fold (geometric mean, 160 versus 4 µg/g; P
AB - In nephrotic syndrome, plasminogen is aberrantly filtered from plasma to the urinary space and activated along the tubular system. In vitro, plasmin increases ENaC current by proteolytic cleavage of the γ-subunit. It was hypothesized that preeclampsia is associated with plasmin-dependent ability of tubular fluid to activate ENaC. Urine was sampled from 16 preeclamptic (PE) patients and 17 normotensive pregnant women (Ctrl). Urine was analyzed for plasmin(ogen), creatinine, albumin, aldosterone, Na(+), K(+), proteolytic activity, and for its effect on inward current in cortical collecting duct cells (M1 cells) by whole-cell patch clamp. In PE, urine plasmin(ogen): creatinine ratio was elevated 40-fold (geometric mean, 160 versus 4 µg/g; P
U2 - 10.1161/HYPERTENSIONAHA.112.198879
DO - 10.1161/HYPERTENSIONAHA.112.198879
M3 - Journal article
C2 - 22987920
SN - 0194-911X
VL - 60
SP - 1346
EP - 1351
JO - Hypertension
JF - Hypertension
IS - 5
ER -