Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

Casper  Kunstmann-Olsen; Martin Hanczyc; James Hoyland; Steen  Rasmussen; Horst-Günter Rubahn

doi:10.1016/j.snb.2016.01.120

Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

Casper Kunstmann-Olsen
, Martin Hanczyc
, James Hoyland
, Steen Rasmussen
, Horst-Günter Rubahn

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100 μm and 300 μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.

Original language	English
Journal	Sensors and Actuators B: Chemical
Volume	229
Pages (from-to)	7-13
ISSN	0925-4005
DOIs	https://doi.org/10.1016/j.snb.2016.01.120
Publication status	Published - 29. Jan 2016

Keywords

Microfluidic
Flow Cytometry

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title = "Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device",

abstract = "A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100 μm and 300 μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.",

keywords = "Microfluidic, Flow Cytometry",

author = "Casper Kunstmann-Olsen and Martin Hanczyc and James Hoyland and Steen Rasmussen and Horst-G{\"u}nter Rubahn",

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T1 - Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

AU - Kunstmann-Olsen, Casper

AU - Hanczyc, Martin

AU - Hoyland, James

AU - Rasmussen, Steen

AU - Rubahn, Horst-Günter

PY - 2016/1/29

Y1 - 2016/1/29

N2 - A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100 μm and 300 μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.

AB - A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100 μm and 300 μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.

KW - Microfluidic

KW - Flow Cytometry

U2 - 10.1016/j.snb.2016.01.120

DO - 10.1016/j.snb.2016.01.120

M3 - Journal article

SN - 0925-4005

VL - 229

SP - 7

EP - 13

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

Abstract

Keywords

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