Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

Casper Kunstmann-Olsen, Martin Hanczyc, James Hoyland, Steen Rasmussen, Horst-Günter Rubahn

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100 μm and 300 μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.
Original languageEnglish
JournalSensors and Actuators B: Chemical
Volume229
Pages (from-to)7-13
ISSN0925-4005
DOIs
Publication statusPublished - 29. Jan 2016

Keywords

  • Microfluidic
  • Flow Cytometry

Fingerprint

Dive into the research topics of 'Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device'. Together they form a unique fingerprint.

Cite this