UNA (unlocked nucleic acid): A flexible RNA mimic that allows engineering of nucleic acid duplex stability

Niels Langkjær, Anna Pasternak, Jesper Wengel

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

UNA (unlocked nucleic acid) monomers are acyclic derivatives of RNA lacking the C2′–C3′-bond of the ribose ring of RNA. Synthesis of phosphoramidite UNA building blocks of the nucleobases adenine, cytosine, guanine, and uracil is described herein together with their incorporation into RNA strands. UNA monomers additively decrease nucleic acid duplex stability and can be positioned strategically to induce either lack of discrimination of mismatches, that is, universal base behavior, or increased discrimination of mismatches, that is, improved hybridization specificity. UNA-modified RNA duplexes are shown to structurally mimic unmodified RNA duplexes by CD spectroscopy.
Original languageEnglish
JournalBioorganic & Medicinal Chemistry
Volume17
Issue number15
Pages (from-to)5420-5425
Number of pages6
ISSN0968-0896
DOIs
Publication statusPublished - 2009

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