Tumor microenvironmental genomic alterations in juvenile nasopharyngeal angiofibroma

Sara Martoreli Silveira, Maria Aparecida Custódio Domingues, Ossamu Butugan, Maria Mitzi Brentani, Silvia Regina Rogatto

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

BACKGROUND: To better characterize the pathophysiology of juvenile nasopharyngeal angiofibroma (JNA), endothelial and stromal cells were evaluated by genomic imbalances in association with transcript expression levels of genes mapped on these altered regions.

METHODS: High-resolution comparative genomic hybridization (HR-CGH) was used in laser-captured endothelial and stromal cells from 9 JNAs. Ten genes were evaluated by quantitative real-timereverse transcription polymerase chain reaction (qRT-PCR) in 15 cases.

RESULTS: Although gains were more frequently detected in endothelial cells, 57% of chromosomal alterations were common by both components. Gene expression analyses revealed a positive correlation between endothelial and stromal components for ASPM, CDH1, CTNNB1, FGF18, and SUPT16H. A significant difference was found for FGF18 and AURKB overexpression in stromal cells and AR down-expression in endothelial cells.

CONCLUSIONS: A similar pattern of gene expression and chromosomal imbalances in both exponents would suggest a common mechanism of functional regulation. AURKB, FGF18, and SUPT16H were identified as potential molecular markers in JNA.

Original languageEnglish
JournalHead & Neck (Print Edition)
Volume34
Issue number4
Pages (from-to)485-492
ISSN1043-3074
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Adolescent
  • Angiofibroma
  • Chromosome Aberrations
  • Comparative Genomic Hybridization
  • Confidence Intervals
  • Gene Expression Regulation, Neoplastic
  • Genetic Predisposition to Disease
  • Genomic Instability
  • Humans
  • Male
  • Nasopharyngeal Neoplasms
  • Real-Time Polymerase Chain Reaction
  • Sampling Studies
  • Tissue Embedding
  • Tumor Microenvironment
  • Young Adult

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