Treating mouse skull defects with 3D printed fatty acid and tricalcium phosphate implants

Martin Bonde Jensen, Casper Slots, Nicholas Ditzel, Stefanie Hansborg Kolstrup, Moustapha Kassem, Torben Thygesen, Morten Østergaard Andersen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Skull surgery, also known as craniectomy, is done to treat trauma or brain diseases and may require the use of an implant to reestablish skull integrity. This study investigates the performance of 3D printed bone implants in a mouse model of craniectomy with the aim of making biodegradable porous implants that can ultimately be fitted to a patient's anatomy. A nonpolymeric thermoplastic bioink composed of fatty acids and β-tricalcium phosphate was used to 3D print the skull implants. Some of these were sintered to yield pure β-tricalcium phosphate implants. The performance of nonsintered and sintered implants was then compared in two semi-quantitative murine calvarial defect models using computed tomography, histology, and luciferase activity. Both types of implants were biocompatible, but only sintered implants promoted defect healing, with osseointegration to adjacent bone and the formation of new bone and bone marrow tissue in the implant pores. Luciferase scanning and histology showed that mesenchymal stem cells seeded onto the implants engraft and proliferate on the implants after implantation and contribute to forming bone. The experiments indicate that fatty acid-based 3D printing enables the creation of biocompatible and bone-forming β-tricalcium phosphate implants.

Original languageEnglish
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume14
Issue number12
Pages (from-to)1858-1868
ISSN1932-6254
DOIs
Publication statusPublished - Dec 2020

Keywords

  • 3D printing
  • additive manufacturing
  • bone
  • ceramics
  • implants
  • regenerative medicine
  • tissue engineering
  • tricalcium phosphate

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