Tissue distribution of ethanol after intraprostatic injection using a porous needle

Megan N. Eubank; Ján Švihra; Kevin C. DiBona; Matthew Sommers; Tyler Oe; Ján Strnádel; Juraj Miklušica; Peter Szépe; Juraj Marcinek; Benjamin J. King; Mark K. Plante; Ján Ľupták; Mads Hvid Aaberg Poulsen; Masatoshi Kida; Eduard Baco; Ján Švihra; Peter Zvara

doi:10.3389/fonc.2022.1063781

Tissue distribution of ethanol after intraprostatic injection using a porous needle

Megan N. Eubank, Ján Švihra, Kevin C. DiBona, Matthew Sommers, Tyler Oe, Ján Strnádel, Juraj Miklušica, Peter Szépe, Juraj Marcinek, Benjamin J. King, Mark K. Plante, Ján Ľupták, Mads Hvid Aaberg Poulsen, Masatoshi Kida, Eduard Baco, Ján Švihra, Peter Zvara^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Purpose: To develop a safe and precise method for intraprostatic injection, and to establish correlation between the volume of ethanol injectate and the volume of subsequent infiltrated prostate tissue. Materials and methods: We performed intraprostatic injection of 96% ethanol using a needle which has a segment of its wall made of capillary membrane with hundreds of pores in an acute and chronic canine experiment, in heart-beating cadaveric organ donors, and in a xenograft model of human prostate cancer. Whole mount tissue sections were used for three-dimensional reconstruction of the necrotic lesions and calculation of their volumes. Results: The ethanol injection resulted in oval shaped lesions of well-delineated coagulative necrosis. In both healthy human and canine prostates, the prostatic pseudocapsule and neurovascular bundle remained intact without evidence of disruption. There was a linear correlation between administered volume of ethanol and the volume of necrotic lesion. Regression analysis showed strong correlation in the acute canine experiments and in experiments performed on xenografts of human prostate cancer. A formula was calculated for each experiment to estimate the relationship between the injected volume and the volume of infiltrated prostate tissue area. Conclusions: Intraprostatic injection using a porous needle allows for effective and predictable tissue distribution of the injectate in the prostate. Through varying the volume of the agent injected and use of needles with a different length of the porous segment, the volume of infiltrated tissue could be adjusted allowing for targeted focal treatment.

Original language	English
Article number	1063781
Journal	Frontiers in Oncology
Volume	12
ISSN	2234-943X
DOIs	https://doi.org/10.3389/fonc.2022.1063781
Publication status	Published - 4. Jan 2023

Keywords

organ-confined prostate cancer
porous needle
prostate
tissue diffusion
Ultrasound-guided targeted injection

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10.3389/fonc.2022.1063781Licence: CC BY

Open Access VersionFinal published version, 3.23 MBLicence: CC BY

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Eubank, M. N., Švihra, J., DiBona, K. C., Sommers, M., Oe, T., Strnádel, J., Miklušica, J., Szépe, P., Marcinek, J., King, B. J., Plante, M. K., Ľupták, J., Poulsen, M. H. A., Kida, M., Baco, E., Švihra, J., & Zvara, P. (2023). Tissue distribution of ethanol after intraprostatic injection using a porous needle. Frontiers in Oncology, 12, Article 1063781. https://doi.org/10.3389/fonc.2022.1063781

@article{0c80334fae4e45c59ca6af6cbd66347a,

title = "Tissue distribution of ethanol after intraprostatic injection using a porous needle",

abstract = "Purpose: To develop a safe and precise method for intraprostatic injection, and to establish correlation between the volume of ethanol injectate and the volume of subsequent infiltrated prostate tissue. Materials and methods: We performed intraprostatic injection of 96% ethanol using a needle which has a segment of its wall made of capillary membrane with hundreds of pores in an acute and chronic canine experiment, in heart-beating cadaveric organ donors, and in a xenograft model of human prostate cancer. Whole mount tissue sections were used for three-dimensional reconstruction of the necrotic lesions and calculation of their volumes. Results: The ethanol injection resulted in oval shaped lesions of well-delineated coagulative necrosis. In both healthy human and canine prostates, the prostatic pseudocapsule and neurovascular bundle remained intact without evidence of disruption. There was a linear correlation between administered volume of ethanol and the volume of necrotic lesion. Regression analysis showed strong correlation in the acute canine experiments and in experiments performed on xenografts of human prostate cancer. A formula was calculated for each experiment to estimate the relationship between the injected volume and the volume of infiltrated prostate tissue area. Conclusions: Intraprostatic injection using a porous needle allows for effective and predictable tissue distribution of the injectate in the prostate. Through varying the volume of the agent injected and use of needles with a different length of the porous segment, the volume of infiltrated tissue could be adjusted allowing for targeted focal treatment.",

keywords = "organ-confined prostate cancer, porous needle, prostate, tissue diffusion, Ultrasound-guided targeted injection",

author = "Eubank, {Megan N.} and J{\'a}n {\v S}vihra and DiBona, {Kevin C.} and Matthew Sommers and Tyler Oe and J{\'a}n Strn{\'a}del and Juraj Miklu{\v s}ica and Peter Sz{\'e}pe and Juraj Marcinek and King, {Benjamin J.} and Plante, {Mark K.} and J{\'a}n {\v L}upt{\'a}k and Poulsen, {Mads Hvid Aaberg} and Masatoshi Kida and Eduard Baco and J{\'a}n {\v S}vihra and Peter Zvara",

note = "Funding Information: This work was supported by Ministry of Health of the Slovak Republic under the project registration number 2018/5-UKMT-1, Beckett Fund and Odense University Hospital Free Fund. Publisher Copyright: Copyright {\textcopyright} 2023 Eubank, {\v S}vihra, DiBona, Sommers, Oe, Strn{\'a}del, Miklu{\v s}ica, Sz{\'e}pe, Marcinek, King, Plante, {\v L}upt{\'a}k, Poulsen, Kida, Baco, {\v S}vihra and Zvara.",

year = "2023",

month = jan,

day = "4",

doi = "10.3389/fonc.2022.1063781",

language = "English",

volume = "12",

journal = "Frontiers in Oncology",

issn = "2234-943X",

publisher = "Frontiers Media",

}

Eubank, MN, Švihra, J, DiBona, KC, Sommers, M, Oe, T, Strnádel, J, Miklušica, J, Szépe, P, Marcinek, J, King, BJ, Plante, MK, Ľupták, J, Poulsen, MHA, Kida, M, Baco, E, Švihra, J & Zvara, P 2023, 'Tissue distribution of ethanol after intraprostatic injection using a porous needle', Frontiers in Oncology, vol. 12, 1063781. https://doi.org/10.3389/fonc.2022.1063781

TY - JOUR

T1 - Tissue distribution of ethanol after intraprostatic injection using a porous needle

AU - Eubank, Megan N.

AU - Švihra, Ján

AU - DiBona, Kevin C.

AU - Sommers, Matthew

AU - Oe, Tyler

AU - Strnádel, Ján

AU - Miklušica, Juraj

AU - Szépe, Peter

AU - Marcinek, Juraj

AU - King, Benjamin J.

AU - Plante, Mark K.

AU - Ľupták, Ján

AU - Poulsen, Mads Hvid Aaberg

AU - Kida, Masatoshi

AU - Baco, Eduard

AU - Švihra, Ján

AU - Zvara, Peter

N1 - Funding Information: This work was supported by Ministry of Health of the Slovak Republic under the project registration number 2018/5-UKMT-1, Beckett Fund and Odense University Hospital Free Fund. Publisher Copyright: Copyright © 2023 Eubank, Švihra, DiBona, Sommers, Oe, Strnádel, Miklušica, Szépe, Marcinek, King, Plante, Ľupták, Poulsen, Kida, Baco, Švihra and Zvara.

PY - 2023/1/4

Y1 - 2023/1/4

N2 - Purpose: To develop a safe and precise method for intraprostatic injection, and to establish correlation between the volume of ethanol injectate and the volume of subsequent infiltrated prostate tissue. Materials and methods: We performed intraprostatic injection of 96% ethanol using a needle which has a segment of its wall made of capillary membrane with hundreds of pores in an acute and chronic canine experiment, in heart-beating cadaveric organ donors, and in a xenograft model of human prostate cancer. Whole mount tissue sections were used for three-dimensional reconstruction of the necrotic lesions and calculation of their volumes. Results: The ethanol injection resulted in oval shaped lesions of well-delineated coagulative necrosis. In both healthy human and canine prostates, the prostatic pseudocapsule and neurovascular bundle remained intact without evidence of disruption. There was a linear correlation between administered volume of ethanol and the volume of necrotic lesion. Regression analysis showed strong correlation in the acute canine experiments and in experiments performed on xenografts of human prostate cancer. A formula was calculated for each experiment to estimate the relationship between the injected volume and the volume of infiltrated prostate tissue area. Conclusions: Intraprostatic injection using a porous needle allows for effective and predictable tissue distribution of the injectate in the prostate. Through varying the volume of the agent injected and use of needles with a different length of the porous segment, the volume of infiltrated tissue could be adjusted allowing for targeted focal treatment.

AB - Purpose: To develop a safe and precise method for intraprostatic injection, and to establish correlation between the volume of ethanol injectate and the volume of subsequent infiltrated prostate tissue. Materials and methods: We performed intraprostatic injection of 96% ethanol using a needle which has a segment of its wall made of capillary membrane with hundreds of pores in an acute and chronic canine experiment, in heart-beating cadaveric organ donors, and in a xenograft model of human prostate cancer. Whole mount tissue sections were used for three-dimensional reconstruction of the necrotic lesions and calculation of their volumes. Results: The ethanol injection resulted in oval shaped lesions of well-delineated coagulative necrosis. In both healthy human and canine prostates, the prostatic pseudocapsule and neurovascular bundle remained intact without evidence of disruption. There was a linear correlation between administered volume of ethanol and the volume of necrotic lesion. Regression analysis showed strong correlation in the acute canine experiments and in experiments performed on xenografts of human prostate cancer. A formula was calculated for each experiment to estimate the relationship between the injected volume and the volume of infiltrated prostate tissue area. Conclusions: Intraprostatic injection using a porous needle allows for effective and predictable tissue distribution of the injectate in the prostate. Through varying the volume of the agent injected and use of needles with a different length of the porous segment, the volume of infiltrated tissue could be adjusted allowing for targeted focal treatment.

KW - organ-confined prostate cancer

KW - porous needle

KW - prostate

KW - tissue diffusion

KW - Ultrasound-guided targeted injection

U2 - 10.3389/fonc.2022.1063781

DO - 10.3389/fonc.2022.1063781

M3 - Journal article

C2 - 36686794

AN - SCOPUS:85146472230

SN - 2234-943X

VL - 12

JO - Frontiers in Oncology

JF - Frontiers in Oncology

M1 - 1063781

ER -

Tissue distribution of ethanol after intraprostatic injection using a porous needle

Abstract

Keywords

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