There is no hiding if you Seq: recent breakthroughs in Pseudomonas aeruginosa research revealed by genomic andtranscriptomic next-generation sequencing

Richard Xavier Etienne Valli, Mark Lyng, Clare Kirkpatrick*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The advent of next-generation sequencing technology has revolutionized the field of prokaryotic genetics and genomics by allowing interrogation of entire genomes, transcriptomes and global transcription factor binding profiles. As more studies employing these techniques have been performed, the state of the art regarding prokaryotic gene regulation has developed from the level of individual genes to genetic regulatory networks and systems biology. When applied to bacterial pathogens, particularly valuable insights have been gained into the regulation of virulence-associated genes, their relative importance to bacterial survival in planktonic, biofilm or host infection scenarios, antimicrobial resistance and the molecular details of host-pathogen interactions. This review outlines some of the latest developments and applications of next-generation sequencing techniques that have used primarily Pseudomonas aeruginosa as a model system. We focus particularly on insights into Pseudomonas virulence and infection that have been gained from these approaches and the future directions in which this field could develop.

Original languageEnglish
JournalJournal of Medical Microbiology
Volume69
Issue number2
Pages (from-to)162-175
Number of pages14
ISSN0022-2615
DOIs
Publication statusPublished - Feb 2020

Keywords

  • ChIP-Seq
  • Host-pathogen interaction
  • Next-generation sequencing
  • Pseudomonas
  • RNA-Seq
  • Transposon insertion sequencing

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