TY - JOUR
T1 - The unexplored role of sedentary time and physical activity in glucose and lipid metabolism-related placental mRNAs in pregnant women who are obese
T2 - the DALI lifestyle randomised controlled trial
AU - Acosta-Manzano, Pedro
AU - Leopold-Posch, Barbara
AU - Simmons, David
AU - Devlieger, Roland
AU - Galjaard, Sander
AU - Corcoy, Rosa
AU - Adelantado, Juan M
AU - Dunne, Fidelma
AU - Harreiter, Jürgen
AU - Kautzky-Willer, Alexandra
AU - Damm, Peter
AU - Mathiesen, Elisabeth R
AU - Jensen, Dorte M
AU - Andersen, Lise-Lotte
AU - Tanvig, Mette
AU - Lapolla, Annunziata
AU - Dalfra, Maria-Grazia
AU - Bertolotto, Allessandra
AU - Wender-Ozegowska, Ewa
AU - Zawiejska, Agnieszka
AU - Hill, David J
AU - Snoek, Frank J
AU - Jelsma, Judith G M
AU - Desoye, Gernot
AU - van Poppel, Mireille N M
N1 - This article is protected by copyright. All rights reserved.
PY - 2022/4
Y1 - 2022/4
N2 - OBJECTIVE: We aimed to explore: (i) the association of sedentary time (ST) and physical activity (PA) during pregnancy with the placental expression of genes related to glucose and lipid metabolism in pregnant women who are obese; (ii) maternal metabolic factors mediating changes in these placental transcripts; and (iii) cord blood markers related to the mRNAs mediating neonatal adiposity.DESIGN: Multicentre randomised controlled trial.SETTING: Hospitals in nine European countries.POPULATION: A cohort of 112 pregnant women with placental tissue.METHODS: Both ST and moderate-to-vigorous PA (MVPA) levels were measured objectively using accelerometry at three time periods during pregnancy.MAIN OUTCOME MEASURES: Placental mRNAs (FATP2, FATP3, FABP4, GLUT1 and PPAR-γ) were measured with NanoString technology. Maternal and fetal metabolic markers and neonatal adiposity were assessed.RESULTS: Longer periods of ST, especially in early to middle pregnancy, was associated with lower placental FATP2 and FATP3 expression (P < 0.05), whereas MVPA at baseline was inversely associated with GLUT1 mRNA (P = 0.02). Although placental FATP2 and FATP3 expression were regulated by the insulin-glucose axis (P < 0.05), no maternal metabolic marker mediated the association of ST/MVPA with placental mRNAs (P > 0.05). Additionally, placental FATP2 expression was inversely associated with cord blood triglycerides and free fatty acids (FFAs; P < 0.01). No cord blood marker mediated neonatal adiposity except for cord blood leptin, which mediated the effects of PPAR-γ on neonatal sum of skinfolds (P < 0.05).CONCLUSIONS: In early to middle pregnancy, ST is associated with the expression of placental genes linked to lipid transport. PA is hardly related to transporter mRNAs. Strategies aimed at reducing sedentary behaviour during pregnancy could modulate placental gene expression, which may help to prevent unfavourable fetal and maternal pregnancy outcomes.TWEETABLE ABSTRACT: Reducing sedentary behaviour in pregnancy might modulate placental expression of genes related to lipid metabolism in women who are obese.
AB - OBJECTIVE: We aimed to explore: (i) the association of sedentary time (ST) and physical activity (PA) during pregnancy with the placental expression of genes related to glucose and lipid metabolism in pregnant women who are obese; (ii) maternal metabolic factors mediating changes in these placental transcripts; and (iii) cord blood markers related to the mRNAs mediating neonatal adiposity.DESIGN: Multicentre randomised controlled trial.SETTING: Hospitals in nine European countries.POPULATION: A cohort of 112 pregnant women with placental tissue.METHODS: Both ST and moderate-to-vigorous PA (MVPA) levels were measured objectively using accelerometry at three time periods during pregnancy.MAIN OUTCOME MEASURES: Placental mRNAs (FATP2, FATP3, FABP4, GLUT1 and PPAR-γ) were measured with NanoString technology. Maternal and fetal metabolic markers and neonatal adiposity were assessed.RESULTS: Longer periods of ST, especially in early to middle pregnancy, was associated with lower placental FATP2 and FATP3 expression (P < 0.05), whereas MVPA at baseline was inversely associated with GLUT1 mRNA (P = 0.02). Although placental FATP2 and FATP3 expression were regulated by the insulin-glucose axis (P < 0.05), no maternal metabolic marker mediated the association of ST/MVPA with placental mRNAs (P > 0.05). Additionally, placental FATP2 expression was inversely associated with cord blood triglycerides and free fatty acids (FFAs; P < 0.01). No cord blood marker mediated neonatal adiposity except for cord blood leptin, which mediated the effects of PPAR-γ on neonatal sum of skinfolds (P < 0.05).CONCLUSIONS: In early to middle pregnancy, ST is associated with the expression of placental genes linked to lipid transport. PA is hardly related to transporter mRNAs. Strategies aimed at reducing sedentary behaviour during pregnancy could modulate placental gene expression, which may help to prevent unfavourable fetal and maternal pregnancy outcomes.TWEETABLE ABSTRACT: Reducing sedentary behaviour in pregnancy might modulate placental expression of genes related to lipid metabolism in women who are obese.
KW - Fatty acid
KW - fetal development
KW - gene expression
KW - gestation
KW - gestational diabetes mellitus
KW - nutrient transport
KW - placental development
KW - Life Style
KW - Humans
KW - Placenta/metabolism
KW - Glucose
KW - Pregnancy
KW - Exercise
KW - Pregnant Women
KW - Obesity/complications
KW - Female
KW - Lipid Metabolism/genetics
KW - RNA, Messenger
KW - Infant, Newborn
KW - Pregnancy Outcome
KW - Sedentary Behavior
U2 - 10.1111/1471-0528.16945
DO - 10.1111/1471-0528.16945
M3 - Journal article
C2 - 34559946
SN - 1470-0328
VL - 129
SP - 708
EP - 721
JO - BJOG: An International Journal of Obstetrics and Gynaecology
JF - BJOG: An International Journal of Obstetrics and Gynaecology
IS - 5
ER -