The regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase

Xun Shi, Juan Li, Xiaoju Zou, Joel Greggain, Steven V Rodkaer, Nils J. Færgeman, Bin Liang, Jennifer L Watts

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Fatty acid desaturation regulates membrane function and fat storage in animals. To determine the contribution of stearoyl-CoA desaturase (SCD) activity on fat storage and development in the nematode Caenorhabditis elegans, we analyzed the lipid composition and lipid droplet size in the fat-6;fat-7 desaturase mutants, independently, and in combination with mutants disrupted in conserved lipid metabolic pathways. C. elegans with impaired SCD activity displayed both reduced fat stores and decreased lipid droplet size. Mutants in the daf-2 (insulin-like growth factor receptor), rsks-1 (homolog of p70S6kinase, an effector of the TOR signaling pathway), and daf-7 (transforming growth factor beta) display high fat stores, the opposite of the low fat observed in the fat-6;fat-7 desaturase mutants. The metabolic mutants in combination with fat-6;fat-7 displayed low fat stores, with the exception of the daf-2;fat-6;fat-7 triple mutants, which had increased de novo fatty acid synthesis and wild type levels of fat stores. Notably, stearoyl-CoA desaturase activity is required for the formation of large-sized lipid droplets in all mutant backgrounds, as well as for normal ratios of phosphatidylcholine (PC) to phosphatidylethanolamine (PE). These studies reveal previously uncharacterized roles for stearoyl-CoA desaturases in the regulation of lipid droplet size and membrane phospholipid composition.
Original languageEnglish
JournalJournal of Lipid Research
Volume54
Pages (from-to)2504-2514
ISSN0022-2275
DOIs
Publication statusPublished - 2013

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Stearoyl-CoA Desaturase
Phospholipids
Fats
Lipids
Chemical analysis
Caenorhabditis elegans
Lipid Droplets
Fatty Acids
Membranes
Somatomedin Receptors

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Shi, Xun ; Li, Juan ; Zou, Xiaoju ; Greggain, Joel ; Rodkaer, Steven V ; Færgeman, Nils J. ; Liang, Bin ; Watts, Jennifer L. / The regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase. In: Journal of Lipid Research. 2013 ; Vol. 54. pp. 2504-2514.
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abstract = "Fatty acid desaturation regulates membrane function and fat storage in animals. To determine the contribution of stearoyl-CoA desaturase (SCD) activity on fat storage and development in the nematode Caenorhabditis elegans, we analyzed the lipid composition and lipid droplet size in the fat-6;fat-7 desaturase mutants, independently, and in combination with mutants disrupted in conserved lipid metabolic pathways. C. elegans with impaired SCD activity displayed both reduced fat stores and decreased lipid droplet size. Mutants in the daf-2 (insulin-like growth factor receptor), rsks-1 (homolog of p70S6kinase, an effector of the TOR signaling pathway), and daf-7 (transforming growth factor beta) display high fat stores, the opposite of the low fat observed in the fat-6;fat-7 desaturase mutants. The metabolic mutants in combination with fat-6;fat-7 displayed low fat stores, with the exception of the daf-2;fat-6;fat-7 triple mutants, which had increased de novo fatty acid synthesis and wild type levels of fat stores. Notably, stearoyl-CoA desaturase activity is required for the formation of large-sized lipid droplets in all mutant backgrounds, as well as for normal ratios of phosphatidylcholine (PC) to phosphatidylethanolamine (PE). These studies reveal previously uncharacterized roles for stearoyl-CoA desaturases in the regulation of lipid droplet size and membrane phospholipid composition.",
author = "Xun Shi and Juan Li and Xiaoju Zou and Joel Greggain and Rodkaer, {Steven V} and F{\ae}rgeman, {Nils J.} and Bin Liang and Watts, {Jennifer L}",
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The regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase. / Shi, Xun; Li, Juan; Zou, Xiaoju; Greggain, Joel; Rodkaer, Steven V; Færgeman, Nils J.; Liang, Bin; Watts, Jennifer L.

In: Journal of Lipid Research, Vol. 54, 2013, p. 2504-2514.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - The regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase

AU - Shi, Xun

AU - Li, Juan

AU - Zou, Xiaoju

AU - Greggain, Joel

AU - Rodkaer, Steven V

AU - Færgeman, Nils J.

AU - Liang, Bin

AU - Watts, Jennifer L

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N2 - Fatty acid desaturation regulates membrane function and fat storage in animals. To determine the contribution of stearoyl-CoA desaturase (SCD) activity on fat storage and development in the nematode Caenorhabditis elegans, we analyzed the lipid composition and lipid droplet size in the fat-6;fat-7 desaturase mutants, independently, and in combination with mutants disrupted in conserved lipid metabolic pathways. C. elegans with impaired SCD activity displayed both reduced fat stores and decreased lipid droplet size. Mutants in the daf-2 (insulin-like growth factor receptor), rsks-1 (homolog of p70S6kinase, an effector of the TOR signaling pathway), and daf-7 (transforming growth factor beta) display high fat stores, the opposite of the low fat observed in the fat-6;fat-7 desaturase mutants. The metabolic mutants in combination with fat-6;fat-7 displayed low fat stores, with the exception of the daf-2;fat-6;fat-7 triple mutants, which had increased de novo fatty acid synthesis and wild type levels of fat stores. Notably, stearoyl-CoA desaturase activity is required for the formation of large-sized lipid droplets in all mutant backgrounds, as well as for normal ratios of phosphatidylcholine (PC) to phosphatidylethanolamine (PE). These studies reveal previously uncharacterized roles for stearoyl-CoA desaturases in the regulation of lipid droplet size and membrane phospholipid composition.

AB - Fatty acid desaturation regulates membrane function and fat storage in animals. To determine the contribution of stearoyl-CoA desaturase (SCD) activity on fat storage and development in the nematode Caenorhabditis elegans, we analyzed the lipid composition and lipid droplet size in the fat-6;fat-7 desaturase mutants, independently, and in combination with mutants disrupted in conserved lipid metabolic pathways. C. elegans with impaired SCD activity displayed both reduced fat stores and decreased lipid droplet size. Mutants in the daf-2 (insulin-like growth factor receptor), rsks-1 (homolog of p70S6kinase, an effector of the TOR signaling pathway), and daf-7 (transforming growth factor beta) display high fat stores, the opposite of the low fat observed in the fat-6;fat-7 desaturase mutants. The metabolic mutants in combination with fat-6;fat-7 displayed low fat stores, with the exception of the daf-2;fat-6;fat-7 triple mutants, which had increased de novo fatty acid synthesis and wild type levels of fat stores. Notably, stearoyl-CoA desaturase activity is required for the formation of large-sized lipid droplets in all mutant backgrounds, as well as for normal ratios of phosphatidylcholine (PC) to phosphatidylethanolamine (PE). These studies reveal previously uncharacterized roles for stearoyl-CoA desaturases in the regulation of lipid droplet size and membrane phospholipid composition.

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DO - 10.1194/jlr.M039669

M3 - Journal article

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JO - Journal of Lipid Research

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