The rates and players of denitrification, dissimilatory nitrate reduction to ammonia (DNRA) and anaerobic ammonia oxidation (anammox) in mangrove soils

Danice M Luvizotto, Juliana E Araujo, Michele DE Cássia P Silva, Armando C F Dias, Beate Kraft, Halina Tegetmeye, Marc Strous, Fernando D Andreote*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Mangroves are ecosystems located in the transition zone between land and sea, characterized by periodic flooding that confer to its unique characteristics. Little is known about the transformation of nutrients that occur during the organic matter degradation in this system. In this study, we monitor the nitrogen transformations in soils from three mangroves with distinct levels of contamination using labeled 15NO3-. We also screened the mangroves metagenomes for the presence of genes that encode enzymes involved in denitrification (nirS, nirK, nosZ, norB and narG), anaerobic oxidation of ammonia (anammox) (hh, hao and hzo) and dissimilatory nitrate reduction to ammonium (DNRA) (nrfA). The transformations of 15NO3- indicated the balance of denitrification over anammox and DNRA in all three mangroves, with lower rates of processes in the mangrove affected by oil contamination. The metagenomic analysis detected 56 sequences related to denitrification, 19 with anammox and 6 with DNRA. Genes related with denitrification were phylogenetically distributed among several groups of bacteria (mainly Gammaproteobacteria). Anammox and DNRA related sequences were affiliated with Planctomycetes and Gammaproteobacteria, respectively. Thus, metagenomic and functional approaches supported the description of denitrification, anammox and DNRA rates in mangrove soils, and identified the major bacterial groups involved in these processes.

Original languageEnglish
Article numbere20180373
JournalAnais da Academia Brasileira de Ciencias.
Volume91
Issue numberSuppl. 1
Number of pages14
ISSN0001-3765
DOIs
Publication statusPublished - 2019

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Ammonia
Metagenomics
Metagenome
Ammonium Compounds
Ecosystem
Oils
Nitrogen
Food
Enzymes

Keywords

  • Anaerobiosis
  • Environmental contamination
  • Metagenome
  • N cycling
  • Nitrates/metabolism
  • Oxidation-Reduction
  • Ammonia/metabolism
  • Denitrification/genetics
  • Wetlands
  • Nitrogen Cycle
  • Metagenome/genetics
  • Soil
  • Plants/metabolism

Cite this

Luvizotto, Danice M ; Araujo, Juliana E ; Silva, Michele DE Cássia P ; Dias, Armando C F ; Kraft, Beate ; Tegetmeye, Halina ; Strous, Marc ; Andreote, Fernando D. / The rates and players of denitrification, dissimilatory nitrate reduction to ammonia (DNRA) and anaerobic ammonia oxidation (anammox) in mangrove soils. In: Anais da Academia Brasileira de Ciencias. 2019 ; Vol. 91, No. Suppl. 1.
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abstract = "Mangroves are ecosystems located in the transition zone between land and sea, characterized by periodic flooding that confer to its unique characteristics. Little is known about the transformation of nutrients that occur during the organic matter degradation in this system. In this study, we monitor the nitrogen transformations in soils from three mangroves with distinct levels of contamination using labeled 15NO3-. We also screened the mangroves metagenomes for the presence of genes that encode enzymes involved in denitrification (nirS, nirK, nosZ, norB and narG), anaerobic oxidation of ammonia (anammox) (hh, hao and hzo) and dissimilatory nitrate reduction to ammonium (DNRA) (nrfA). The transformations of 15NO3- indicated the balance of denitrification over anammox and DNRA in all three mangroves, with lower rates of processes in the mangrove affected by oil contamination. The metagenomic analysis detected 56 sequences related to denitrification, 19 with anammox and 6 with DNRA. Genes related with denitrification were phylogenetically distributed among several groups of bacteria (mainly Gammaproteobacteria). Anammox and DNRA related sequences were affiliated with Planctomycetes and Gammaproteobacteria, respectively. Thus, metagenomic and functional approaches supported the description of denitrification, anammox and DNRA rates in mangrove soils, and identified the major bacterial groups involved in these processes.",
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The rates and players of denitrification, dissimilatory nitrate reduction to ammonia (DNRA) and anaerobic ammonia oxidation (anammox) in mangrove soils. / Luvizotto, Danice M; Araujo, Juliana E; Silva, Michele DE Cássia P; Dias, Armando C F; Kraft, Beate; Tegetmeye, Halina; Strous, Marc; Andreote, Fernando D.

In: Anais da Academia Brasileira de Ciencias., Vol. 91, No. Suppl. 1, e20180373, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - The rates and players of denitrification, dissimilatory nitrate reduction to ammonia (DNRA) and anaerobic ammonia oxidation (anammox) in mangrove soils

AU - Luvizotto, Danice M

AU - Araujo, Juliana E

AU - Silva, Michele DE Cássia P

AU - Dias, Armando C F

AU - Kraft, Beate

AU - Tegetmeye, Halina

AU - Strous, Marc

AU - Andreote, Fernando D

PY - 2019

Y1 - 2019

N2 - Mangroves are ecosystems located in the transition zone between land and sea, characterized by periodic flooding that confer to its unique characteristics. Little is known about the transformation of nutrients that occur during the organic matter degradation in this system. In this study, we monitor the nitrogen transformations in soils from three mangroves with distinct levels of contamination using labeled 15NO3-. We also screened the mangroves metagenomes for the presence of genes that encode enzymes involved in denitrification (nirS, nirK, nosZ, norB and narG), anaerobic oxidation of ammonia (anammox) (hh, hao and hzo) and dissimilatory nitrate reduction to ammonium (DNRA) (nrfA). The transformations of 15NO3- indicated the balance of denitrification over anammox and DNRA in all three mangroves, with lower rates of processes in the mangrove affected by oil contamination. The metagenomic analysis detected 56 sequences related to denitrification, 19 with anammox and 6 with DNRA. Genes related with denitrification were phylogenetically distributed among several groups of bacteria (mainly Gammaproteobacteria). Anammox and DNRA related sequences were affiliated with Planctomycetes and Gammaproteobacteria, respectively. Thus, metagenomic and functional approaches supported the description of denitrification, anammox and DNRA rates in mangrove soils, and identified the major bacterial groups involved in these processes.

AB - Mangroves are ecosystems located in the transition zone between land and sea, characterized by periodic flooding that confer to its unique characteristics. Little is known about the transformation of nutrients that occur during the organic matter degradation in this system. In this study, we monitor the nitrogen transformations in soils from three mangroves with distinct levels of contamination using labeled 15NO3-. We also screened the mangroves metagenomes for the presence of genes that encode enzymes involved in denitrification (nirS, nirK, nosZ, norB and narG), anaerobic oxidation of ammonia (anammox) (hh, hao and hzo) and dissimilatory nitrate reduction to ammonium (DNRA) (nrfA). The transformations of 15NO3- indicated the balance of denitrification over anammox and DNRA in all three mangroves, with lower rates of processes in the mangrove affected by oil contamination. The metagenomic analysis detected 56 sequences related to denitrification, 19 with anammox and 6 with DNRA. Genes related with denitrification were phylogenetically distributed among several groups of bacteria (mainly Gammaproteobacteria). Anammox and DNRA related sequences were affiliated with Planctomycetes and Gammaproteobacteria, respectively. Thus, metagenomic and functional approaches supported the description of denitrification, anammox and DNRA rates in mangrove soils, and identified the major bacterial groups involved in these processes.

KW - Anaerobiosis

KW - Environmental contamination

KW - Metagenome

KW - N cycling

KW - Nitrates/metabolism

KW - Oxidation-Reduction

KW - Ammonia/metabolism

KW - Denitrification/genetics

KW - Wetlands

KW - Nitrogen Cycle

KW - Metagenome/genetics

KW - Soil

KW - Plants/metabolism

U2 - 10.1590/0001-3765201820180373

DO - 10.1590/0001-3765201820180373

M3 - Journal article

C2 - 30379272

VL - 91

JO - Anais da Academia Brasileira de Ciencias.

JF - Anais da Academia Brasileira de Ciencias.

SN - 0001-3765

IS - Suppl. 1

M1 - e20180373

ER -