The possibility of discriminating within the Bacillus cereus group using gyrB sequencing and PCR-RFLP

Gert B Jensen*, Niels Fisker, Thomas Sparsø, Lars Andrup

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Based on a combination of PCR and restriction endonuclease (RE) digestion (PCR-RE digestion), we have examined the possibility of differentiating members of the Bacillus cereus group. Fragments of the gyrB gene (362 bp) from pure cultures of 12 B. cereus, 25 B. thuringiensis, 25 B. mycoides and two B. anthracis strains were amplified and subsequently digested with Sau3A1. Furthermore, a majority of the amplicons were sequenced directly to verify the PCR-RE results. The results obtained suggest that only the B. mycoides generates specific fragments following PCR-RE. Conversely, it was not possible to discriminate between the B. cereus and the B. thuringiensis strains using the methods described.

Original languageEnglish
JournalInternational Journal of Food Microbiology
Volume104
Issue number1
Pages (from-to)113-120
ISSN0168-1605
DOIs
Publication statusPublished - 25. Sept 2005
Externally publishedYes

Keywords

  • Bacillus cereus
  • Base Sequence
  • DNA Gyrase
  • DNA Restriction Enzymes
  • DNA, Bacterial
  • Food Microbiology
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Restriction Mapping
  • Species Specificity

Fingerprint

Dive into the research topics of 'The possibility of discriminating within the Bacillus cereus group using gyrB sequencing and PCR-RFLP'. Together they form a unique fingerprint.

Cite this