The performance of GeneXpert® PCR assay in detecting group B streptococcus colonization at labor

Mohammed Rohi Khalil*, Jens Kjolseth Moller, Stine Yde Nielsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Background: The aim of this study was to assess the performance of GeneXpertR group B streptococcus (GBS) PCR assays in a Danish setting, using rectovaginal GBS culture at labor as the gold standard. Methods: Three hundred and sixty-six (366) women with one or more of the following risk factors for GBS carriage.GBS during current pregnancy, prior infant with EOGBS, temperature >38.0 °C during labor, preterm labor <37 weeks of gestation, rupture of membranes ≥18 hours.were included in the study. Intrapartum rectovaginal swab samples were tested at the bedside by GeneXpertR GBS PCR assay, and cultured on agar plates (Granada) with and without prior use of growth-selective enrichment broth. Results: The GeneXpertR GBS PCR assay showed a sensitivity of 91.7%, a specificity of 97.2%, a PPV of 92.6 %, and a NPV of 96.8%. The turnaround time of the assay was 50 minutes. Conclusions: The GeneXpertR has a high performance, indicating that the assay can be used in a clinical setting.

Original languageEnglish
JournalClinical and Experimental Obstetrics and Gynecology
Volume48
Issue number3
Pages (from-to)661-664
ISSN0390-6663
DOIs
Publication statusPublished - 15. Jun 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s). Published by IMR Press.

Keywords

  • Early-onset neonatal infection
  • GeneXpert®
  • Group B streptococcus
  • Polymerase chain reaction
  • Rapid intrapartum assay

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