The effects of storage on platelet function in different blood products

Signe Sperling, Pernille J. Vinholt, Ulrik Sprogøe, Mark H. Yazer, Henrik Frederiksen, Christian Nielsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Objectives: Reduced platelet (PLT) function during storage has been shown for buffy-coat-derived platelet concentrates (BCP) and apheresis platelet units (AP), while for whole blood (WB) it has not been well studied. The aim of this study was to investigate PLT function in these blood products throughout storage using a novel flow cytometric assay. Methods: Flow cytometric measurement of agonist-induced platelet aggregation, CD62P expression and PAC-1 binding during storage in BCP, AP (1–9 days at 20°C) and WB (1–21 days at 2–6°C). Results: PLT-aggregation capacity decreased from day 1 to day 7 for almost all product–agonist combinations (P =.004 to P =.029) with aggregation capacity of WB being similar to that of AP and BCP. WB aggregation capacity remained relatively unchanged from day 7 to day 21. For all blood products, the fraction of agonist-induced CD62P-expression remained high and the fraction of PAC-1 binding decreased during storage. WB PLTs underwent only small changes in CD62P expression and PAC-1 binding from day 7 to day 21. Conclusion: This study found PLT aggregation in WB stored at 4°C to be as least as good as for BCP and AP stored at 20°C. WB retained significant PLT-aggregation capacity to day 21.

Original languageEnglish
JournalHematology
Volume24
Issue number1
Pages (from-to)89-96
ISSN1024-5332
DOIs
Publication statusPublished - 2019

Keywords

  • apheresis platelet unit
  • buffy-coat-derived platelet concentrate
  • flow cytometry
  • platelet activation
  • Platelet aggregation capacity
  • whole blood
  • P-Selectin/metabolism
  • Blood Platelets/cytology
  • Blood Preservation
  • Dual Specificity Phosphatase 2/metabolism
  • Humans
  • Platelet Aggregation
  • Flow Cytometry
  • Time Factors

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