The activity of nitrifying microorganisms in a high-altitude Andean wetland

Veronica Molina, Cristina Dorador, Camila Fernandez, Laura Bristow, Yoanna Eissler, Martha Hengst, Klaudia Hernandez, Lasse Mork Olsen, Chris Harrod, Francisca Marchant, Cristobal Anguita, Marcela Cornejo

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

High-altitude wetland holds freshwater springs, evaporitic ponds and lagoon with variable salinity and nutrients, potentially influencing the ecology of nitrifying communities. In this study, nitrifying microorganisms in Salar de Huasco (Chile) were surveyed to determine bacterial and archaeal contribution to ammonium (AO), nitrite oxidation (NO), ammonium uptake (AU) during wet and dry seasons. The activity signals from these groups were assessed by specific amoA-qPCR transcription, 15N tracer studies and addition of group specific inhibitor experiments for nitrifying microorganisms (N1-guanyl-1, 7-diaminoheptane [GC7]-archaeal specific and allylthiourea [ATU]-bacterial specific). Nitrifying communities, i.e. Nitrosopumilus, Nitrosospira, Nitrosomonas, Kuenenia and Nitrospira, were more frequent (∼0.25% of 16S rRNA sequences) at low salinity sites. Bacterial amoA-qPCR transcripts also increased at low salinity and along in situ ammonium increase observed between wet/dry seasons. Nutrient changes through time and 15N tracer experiments results showed that AO and NO were detected and peaked mainly at low salinity-high ammonium sites (<37 000 μS cm-1 and >0.3 μM), whereas AU was predominant at evaporitic sites. Our results indicate that salinity and ammonium affect the nitrifying communities that are potentially more active at low-salinity sites but persistent at saltier evaporitic areas of the wetland when ammonium is available.

Original languageEnglish
JournalFEMS Microbiology Ecology
Volume94
Issue number6
ISSN0168-6496
DOIs
Publication statusPublished - Jun 2018

Fingerprint

Wetlands
Ammonium Compounds
ammonium
microorganism
wetland
salinity
wet season
nitrite
dry season
tracer
oxidation
Food
nutrient
Chile
Ecology
Fresh Water
inhibitor
lagoon
pond
experiment

Keywords

  • ammonia-oxidation
  • nitrite-oxidation
  • AOB
  • AOA
  • nitrifier diversity
  • salinity
  • Oxidation-Reduction
  • Bacteria/genetics
  • Ammonia/metabolism
  • Fresh Water/microbiology
  • RNA, Ribosomal, 16S/genetics
  • Phylogeny
  • Salinity
  • Wetlands
  • Nitrification/physiology
  • Archaea/genetics
  • Chile
  • Nitrites/metabolism
  • Altitude
  • Ammonium Compounds/metabolism

Cite this

Molina, V., Dorador, C., Fernandez, C., Bristow, L., Eissler, Y., Hengst, M., ... Cornejo, M. (2018). The activity of nitrifying microorganisms in a high-altitude Andean wetland. FEMS Microbiology Ecology, 94(6). https://doi.org/10.1093/femsec/fiy062
Molina, Veronica ; Dorador, Cristina ; Fernandez, Camila ; Bristow, Laura ; Eissler, Yoanna ; Hengst, Martha ; Hernandez, Klaudia ; Olsen, Lasse Mork ; Harrod, Chris ; Marchant, Francisca ; Anguita, Cristobal ; Cornejo, Marcela. / The activity of nitrifying microorganisms in a high-altitude Andean wetland. In: FEMS Microbiology Ecology. 2018 ; Vol. 94, No. 6.
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title = "The activity of nitrifying microorganisms in a high-altitude Andean wetland",
abstract = "High-altitude wetland holds freshwater springs, evaporitic ponds and lagoon with variable salinity and nutrients, potentially influencing the ecology of nitrifying communities. In this study, nitrifying microorganisms in Salar de Huasco (Chile) were surveyed to determine bacterial and archaeal contribution to ammonium (AO), nitrite oxidation (NO), ammonium uptake (AU) during wet and dry seasons. The activity signals from these groups were assessed by specific amoA-qPCR transcription, 15N tracer studies and addition of group specific inhibitor experiments for nitrifying microorganisms (N1-guanyl-1, 7-diaminoheptane [GC7]-archaeal specific and allylthiourea [ATU]-bacterial specific). Nitrifying communities, i.e. Nitrosopumilus, Nitrosospira, Nitrosomonas, Kuenenia and Nitrospira, were more frequent (∼0.25{\%} of 16S rRNA sequences) at low salinity sites. Bacterial amoA-qPCR transcripts also increased at low salinity and along in situ ammonium increase observed between wet/dry seasons. Nutrient changes through time and 15N tracer experiments results showed that AO and NO were detected and peaked mainly at low salinity-high ammonium sites (<37 000 μS cm-1 and >0.3 μM), whereas AU was predominant at evaporitic sites. Our results indicate that salinity and ammonium affect the nitrifying communities that are potentially more active at low-salinity sites but persistent at saltier evaporitic areas of the wetland when ammonium is available.",
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author = "Veronica Molina and Cristina Dorador and Camila Fernandez and Laura Bristow and Yoanna Eissler and Martha Hengst and Klaudia Hernandez and Olsen, {Lasse Mork} and Chris Harrod and Francisca Marchant and Cristobal Anguita and Marcela Cornejo",
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Molina, V, Dorador, C, Fernandez, C, Bristow, L, Eissler, Y, Hengst, M, Hernandez, K, Olsen, LM, Harrod, C, Marchant, F, Anguita, C & Cornejo, M 2018, 'The activity of nitrifying microorganisms in a high-altitude Andean wetland', FEMS Microbiology Ecology, vol. 94, no. 6. https://doi.org/10.1093/femsec/fiy062

The activity of nitrifying microorganisms in a high-altitude Andean wetland. / Molina, Veronica; Dorador, Cristina; Fernandez, Camila; Bristow, Laura; Eissler, Yoanna; Hengst, Martha; Hernandez, Klaudia; Olsen, Lasse Mork; Harrod, Chris; Marchant, Francisca; Anguita, Cristobal; Cornejo, Marcela.

In: FEMS Microbiology Ecology, Vol. 94, No. 6, 06.2018.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - The activity of nitrifying microorganisms in a high-altitude Andean wetland

AU - Molina, Veronica

AU - Dorador, Cristina

AU - Fernandez, Camila

AU - Bristow, Laura

AU - Eissler, Yoanna

AU - Hengst, Martha

AU - Hernandez, Klaudia

AU - Olsen, Lasse Mork

AU - Harrod, Chris

AU - Marchant, Francisca

AU - Anguita, Cristobal

AU - Cornejo, Marcela

PY - 2018/6

Y1 - 2018/6

N2 - High-altitude wetland holds freshwater springs, evaporitic ponds and lagoon with variable salinity and nutrients, potentially influencing the ecology of nitrifying communities. In this study, nitrifying microorganisms in Salar de Huasco (Chile) were surveyed to determine bacterial and archaeal contribution to ammonium (AO), nitrite oxidation (NO), ammonium uptake (AU) during wet and dry seasons. The activity signals from these groups were assessed by specific amoA-qPCR transcription, 15N tracer studies and addition of group specific inhibitor experiments for nitrifying microorganisms (N1-guanyl-1, 7-diaminoheptane [GC7]-archaeal specific and allylthiourea [ATU]-bacterial specific). Nitrifying communities, i.e. Nitrosopumilus, Nitrosospira, Nitrosomonas, Kuenenia and Nitrospira, were more frequent (∼0.25% of 16S rRNA sequences) at low salinity sites. Bacterial amoA-qPCR transcripts also increased at low salinity and along in situ ammonium increase observed between wet/dry seasons. Nutrient changes through time and 15N tracer experiments results showed that AO and NO were detected and peaked mainly at low salinity-high ammonium sites (<37 000 μS cm-1 and >0.3 μM), whereas AU was predominant at evaporitic sites. Our results indicate that salinity and ammonium affect the nitrifying communities that are potentially more active at low-salinity sites but persistent at saltier evaporitic areas of the wetland when ammonium is available.

AB - High-altitude wetland holds freshwater springs, evaporitic ponds and lagoon with variable salinity and nutrients, potentially influencing the ecology of nitrifying communities. In this study, nitrifying microorganisms in Salar de Huasco (Chile) were surveyed to determine bacterial and archaeal contribution to ammonium (AO), nitrite oxidation (NO), ammonium uptake (AU) during wet and dry seasons. The activity signals from these groups were assessed by specific amoA-qPCR transcription, 15N tracer studies and addition of group specific inhibitor experiments for nitrifying microorganisms (N1-guanyl-1, 7-diaminoheptane [GC7]-archaeal specific and allylthiourea [ATU]-bacterial specific). Nitrifying communities, i.e. Nitrosopumilus, Nitrosospira, Nitrosomonas, Kuenenia and Nitrospira, were more frequent (∼0.25% of 16S rRNA sequences) at low salinity sites. Bacterial amoA-qPCR transcripts also increased at low salinity and along in situ ammonium increase observed between wet/dry seasons. Nutrient changes through time and 15N tracer experiments results showed that AO and NO were detected and peaked mainly at low salinity-high ammonium sites (<37 000 μS cm-1 and >0.3 μM), whereas AU was predominant at evaporitic sites. Our results indicate that salinity and ammonium affect the nitrifying communities that are potentially more active at low-salinity sites but persistent at saltier evaporitic areas of the wetland when ammonium is available.

KW - ammonia-oxidation

KW - nitrite-oxidation

KW - AOB

KW - AOA

KW - nitrifier diversity

KW - salinity

KW - Oxidation-Reduction

KW - Bacteria/genetics

KW - Ammonia/metabolism

KW - Fresh Water/microbiology

KW - RNA, Ribosomal, 16S/genetics

KW - Phylogeny

KW - Salinity

KW - Wetlands

KW - Nitrification/physiology

KW - Archaea/genetics

KW - Chile

KW - Nitrites/metabolism

KW - Altitude

KW - Ammonium Compounds/metabolism

U2 - 10.1093/femsec/fiy062

DO - 10.1093/femsec/fiy062

M3 - Journal article

C2 - 29668898

VL - 94

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

SN - 0168-6496

IS - 6

ER -