Tetrameric and polymeric silver complexes of the omeprazole scaffold; synthesis, structure, in vitro and in vivo antimicrobial activities and DNA interaction

Vibe Jakobsen, Livia Viganor, Alfonso Blanco-Fernández, Orla Howe, Michael Devereux, Christine J. McKenzie, Vickie McKee*

*Corresponding author for this work

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Abstract

Two complexes [AgI(pmtbH)]4 (1) and {[Ag4(pmtbH)4(NO3)4·2X}n (2) (where pmtbH is 2-[(2-pyridinylmethyl)thio]-1H-benzimidazole and X is H2O or MeOH) were synthesised and structurally characterised. Complex 2 showed therapeutic potential against Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa but complex 1 did not show significant activity in vitro. Further in vivo studies using larvae of the insect Galleria mellonella indicated that complex 2 significantly stimulates the immune system and that pre-treatment with the complex offers appreciable protection against all three bacteria. Real-time flow cytometry data support the observed antimicrobial profile of complex 2 and suggest the antimicrobial response may be linked to a form of bacterial programmed cell death (PCD). Complex 2 was found to interact with DNA in the bacterial and fungal cells but it did not cleave plasmid DNA isolated from the three bacteria.

Original languageEnglish
JournalJournal of Inorganic Biochemistry
Volume186
Pages (from-to)317-328
ISSN0162-0134
DOIs
Publication statusPublished - 1. Sep 2018

Keywords

  • Antimicrobial
  • Flow cytometry
  • Galleria mellonella
  • Omeprazole
  • Silver(I)

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