Synthetic LNA/DNA nano-scaffolds for highly efficient diagnostics of nucleic acids and autoimmune antibodies

Irina Kira Astakhova*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Abstract

Herein novel fluorescent oligonucleotides for homogeneous (all-in-solution) detection of nucleic acids and autoimmune antibodies (autoantibodies) are described. The probes are prepared by highly efficient copper-catalyzed click chemistry between novel alkyne-modified locked nucleic acid (LNA) strands and a series of fluorescent azides. The multiply labeled fluorescent LNA/DNA probes prepared herein generally display high binding affinity to complementary DNA/RNA, high quantum yields and, hence, high fluorescence brightness values. With the novel fluorescent probes, specific sensing of the monoclonal human autoantibody is achieved. It makes the novel "clickable" LNA/DNA complexes a very promising tool in molecular diagnostics of both nucleic acids and autoantibodies against DNA. The latter are produced under several autoimmune conditions including antiphospholipide syndrome and systemic lupus erythematosus.

Original languageEnglish
Title of host publicationNanotechnology 2014 : Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014
Volume2
PublisherCRC Press
Publication date2014
Pages181-184
ISBN (Print)978-148225827-1
Publication statusPublished - 2014
EventNanotechnology Conference and Expo: NSTI-Nanotech 2014 - Washington, DC, United States
Duration: 15. Jun 201418. Jun 2014

Conference

ConferenceNanotechnology Conference and Expo
CountryUnited States
CityWashington, DC
Period15/06/201418/06/2014

Keywords

  • Biology
  • Diagnostics
  • Medicine
  • Nanotechnology

Fingerprint Dive into the research topics of 'Synthetic LNA/DNA nano-scaffolds for highly efficient diagnostics of nucleic acids and autoimmune antibodies'. Together they form a unique fingerprint.

Cite this