Synthesis, hybridization and fluorescence properties of a 2'-C-pyrene-triazole modified arabino-uridine nucleotide

Pawan Kumar, Pawan K. Sharma, Poul Nielsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A new pyrene-modified nucleotide monomer is introduced, wherein pyrene is attached to the 2'-position of arabino-uridine through a triazolemethyl linker. When the monomer is introduced into oligonucleotides, very stable DNA duplexes and three way junctions are obtained. An oligonucleotide featuring two modifications in the center shows four-fold increase in the intensity of the pyrene excimer signal on hybridization with an RNA target but not with a DNA target. The new nucleotide monomer has potential in DNA invader probes as well as in RNA targeting and detection.

Original languageEnglish
JournalBioorganic & Medicinal Chemistry
Volume25
Issue number7
Pages (from-to)2084–2090
ISSN0968-0896
DOIs
Publication statusPublished - 2017

Keywords

  • Click chemistry
  • Fluorescent probes
  • Pyrene
  • RNA targeting
  • Three way junctions

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