Surfactant Proteins A, B, C and D in the Human Nasal Airway

Associated with Mucosal Glands and Ciliated Epithelium but Absent in Fluid-Phase Secretions and Mucus

Maria Q Gaunsbaek, Anette D Kjeldsen, Viggo Svane-Knudsen, Maiken L Henriksen, Soren Hansen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Aims: To investigate the presence of surfactant protein (SP) A, B, C and D in nasal airways and to determine whether the proteins exert their main functions in nasal secretions or in the deeper layers of the nasal mucosa. Methods: Volunteers were recruited from the Department of ENT Head and Neck Surgery, Odense University Hospital, Denmark. The study included 39 subjects. Nasal mucosal biopsies were analyzed by immunohistochemistry, and bronchoalveolar and nasal lavages, nasal brush biopsies and nasal mucus were analyzed for SP-A, -B, -C and -D by SDS-PAGE and Western blotting. The presence of SP-A and SP-D in the first three samplings were also analyzed by enzyme-linked immunosorbent assay. Results: In nasal mucosal biopsies, SP-A, -B, -C and -D were all demonstrated in the serous acini of the submucosal glands and in the surface epithelium. SP-D was detected in nasal brush biopsies, whereas the other SPs were absent. Moreover, SP-A, -B, -C and -D were absent in nasal lavage and mucus. Conclusion: SP-A, -B, -C and -D exert their protective effect in the ductal epithelium of the submucosal glands rather than in nasal secretions and mucus. Further studies are required to clarify the functions of these proteins in nasal secretory pathways for understanding upper airway diseases. © 2014 S. Karger AG, Basel.

Original languageEnglish
JournalO R L
Volume76
Issue number5
Pages (from-to)288-301
ISSN0301-1569
DOIs
Publication statusPublished - 10. Dec 2014

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Pulmonary Surfactant-Associated Protein D
Pulmonary Surfactant-Associated Protein A
Fluids and Secretions
Mucus
Nose
Epithelium
IgA receptor
Nasal Mucosa
Denmark
Volunteers
Proteins

Cite this

@article{c4a6a19290304e2e87a0e7471d743164,
title = "Surfactant Proteins A, B, C and D in the Human Nasal Airway: Associated with Mucosal Glands and Ciliated Epithelium but Absent in Fluid-Phase Secretions and Mucus",
abstract = "Aims: To investigate the presence of surfactant protein (SP) A, B, C and D in nasal airways and to determine whether the proteins exert their main functions in nasal secretions or in the deeper layers of the nasal mucosa. Methods: Volunteers were recruited from the Department of ENT Head and Neck Surgery, Odense University Hospital, Denmark. The study included 39 subjects. Nasal mucosal biopsies were analyzed by immunohistochemistry, and bronchoalveolar and nasal lavages, nasal brush biopsies and nasal mucus were analyzed for SP-A, -B, -C and -D by SDS-PAGE and Western blotting. The presence of SP-A and SP-D in the first three samplings were also analyzed by enzyme-linked immunosorbent assay. Results: In nasal mucosal biopsies, SP-A, -B, -C and -D were all demonstrated in the serous acini of the submucosal glands and in the surface epithelium. SP-D was detected in nasal brush biopsies, whereas the other SPs were absent. Moreover, SP-A, -B, -C and -D were absent in nasal lavage and mucus. Conclusion: SP-A, -B, -C and -D exert their protective effect in the ductal epithelium of the submucosal glands rather than in nasal secretions and mucus. Further studies are required to clarify the functions of these proteins in nasal secretory pathways for understanding upper airway diseases. {\circledC} 2014 S. Karger AG, Basel.",
author = "Gaunsbaek, {Maria Q} and Kjeldsen, {Anette D} and Viggo Svane-Knudsen and Henriksen, {Maiken L} and Soren Hansen",
year = "2014",
month = "12",
day = "10",
doi = "10.1159/000369143",
language = "English",
volume = "76",
pages = "288--301",
journal = "O R L",
issn = "0301-1569",
publisher = "S. Karger AG",
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}

Surfactant Proteins A, B, C and D in the Human Nasal Airway : Associated with Mucosal Glands and Ciliated Epithelium but Absent in Fluid-Phase Secretions and Mucus. / Gaunsbaek, Maria Q; Kjeldsen, Anette D; Svane-Knudsen, Viggo; Henriksen, Maiken L; Hansen, Soren.

In: O R L, Vol. 76, No. 5, 10.12.2014, p. 288-301.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Surfactant Proteins A, B, C and D in the Human Nasal Airway

T2 - Associated with Mucosal Glands and Ciliated Epithelium but Absent in Fluid-Phase Secretions and Mucus

AU - Gaunsbaek, Maria Q

AU - Kjeldsen, Anette D

AU - Svane-Knudsen, Viggo

AU - Henriksen, Maiken L

AU - Hansen, Soren

PY - 2014/12/10

Y1 - 2014/12/10

N2 - Aims: To investigate the presence of surfactant protein (SP) A, B, C and D in nasal airways and to determine whether the proteins exert their main functions in nasal secretions or in the deeper layers of the nasal mucosa. Methods: Volunteers were recruited from the Department of ENT Head and Neck Surgery, Odense University Hospital, Denmark. The study included 39 subjects. Nasal mucosal biopsies were analyzed by immunohistochemistry, and bronchoalveolar and nasal lavages, nasal brush biopsies and nasal mucus were analyzed for SP-A, -B, -C and -D by SDS-PAGE and Western blotting. The presence of SP-A and SP-D in the first three samplings were also analyzed by enzyme-linked immunosorbent assay. Results: In nasal mucosal biopsies, SP-A, -B, -C and -D were all demonstrated in the serous acini of the submucosal glands and in the surface epithelium. SP-D was detected in nasal brush biopsies, whereas the other SPs were absent. Moreover, SP-A, -B, -C and -D were absent in nasal lavage and mucus. Conclusion: SP-A, -B, -C and -D exert their protective effect in the ductal epithelium of the submucosal glands rather than in nasal secretions and mucus. Further studies are required to clarify the functions of these proteins in nasal secretory pathways for understanding upper airway diseases. © 2014 S. Karger AG, Basel.

AB - Aims: To investigate the presence of surfactant protein (SP) A, B, C and D in nasal airways and to determine whether the proteins exert their main functions in nasal secretions or in the deeper layers of the nasal mucosa. Methods: Volunteers were recruited from the Department of ENT Head and Neck Surgery, Odense University Hospital, Denmark. The study included 39 subjects. Nasal mucosal biopsies were analyzed by immunohistochemistry, and bronchoalveolar and nasal lavages, nasal brush biopsies and nasal mucus were analyzed for SP-A, -B, -C and -D by SDS-PAGE and Western blotting. The presence of SP-A and SP-D in the first three samplings were also analyzed by enzyme-linked immunosorbent assay. Results: In nasal mucosal biopsies, SP-A, -B, -C and -D were all demonstrated in the serous acini of the submucosal glands and in the surface epithelium. SP-D was detected in nasal brush biopsies, whereas the other SPs were absent. Moreover, SP-A, -B, -C and -D were absent in nasal lavage and mucus. Conclusion: SP-A, -B, -C and -D exert their protective effect in the ductal epithelium of the submucosal glands rather than in nasal secretions and mucus. Further studies are required to clarify the functions of these proteins in nasal secretory pathways for understanding upper airway diseases. © 2014 S. Karger AG, Basel.

U2 - 10.1159/000369143

DO - 10.1159/000369143

M3 - Journal article

VL - 76

SP - 288

EP - 301

JO - O R L

JF - O R L

SN - 0301-1569

IS - 5

ER -