Surfactant protein-D, a potential mediator of inflammation in axial spondyloarthritis

Heidi L Munk, Dalia Fakih, Lene Christiansen, Qihua Tan, Anne F Christensen, Leif Ejstrup, Anne G Loft, Kirsten Junker, Kirsten O Kyvik, Rania Jounblat, Uffe Holmskov, Grith L Sorensen, Peter Junker

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Objectives: Surfactant protein-D (SP-D), an innate immune defence molecule of the collectin family, is expressed in lungs and additional extrapulmonary epithelia. SP-D has immune modulatory and anti-microbial effects depending on its oligomerization. The ratio of high molecular weight (HMW): low molecular weight (LMW) SP-D in serum is mainly determined by the Met11Thr polymorphism (SNP rs721917). We aimed to study the SP-D serum level and the molecular size distribution in patients with untreated axial spondyloarthritis (axSpA) as compared with control subjects.

Methods: Thirty-four patients with disease modifier untreated axSpA according to the ASAS criteria, age 19-63 years, disease duration 3.9 (2.2-5.6) years were included. Demographics, smoking habits, HLA-B27 status, ASDAS, BASDAI, BASFI, BASMI and visual analogue scale scores were recorded. SP-D in serum was measured by ELISA. DNA was isolated from whole blood and single nucleotide polymorphism rs721917 was genotyped. SP-D molecular size distribution was determined using gel filtration chromatography.

Results: SP-D in serum did not differ between patients with axSpA and healthy controls, 1177 (869, 1536) vs 910 (494, 1682) (P = 0.35) and SP-D did not correlate with disease activity. However, the HMW/LMW ratio of SP-D in serum was significantly lower in axSpA, 0.38 (0.18, 0.53) compared with controls 1.49 (0.37, 3.24) when adjusting for the Met11Thr polymorphism, gender, age, BMI and smoking (P = 0.0004). There was no correlation between HMW/LMW ratio and CRP or composite diseases outcome measures.

Conclusion: We suggest that predominance of LMW oligomeric variants of SP-D may enhance local or systemic inflammatory responses in axSpA.

Original languageEnglish
JournalRheumatology (Oxford, England)
Volume57
Issue number10
Pages (from-to)1861–1865
ISSN1462-0324
DOIs
Publication statusPublished - 1. Oct 2018

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Pulmonary Surfactant-Associated Protein D
Molecular Weight
Serum
Single Nucleotide Polymorphism
Smoking
HLA-B27 Antigen
Visual Analog Scale
Habits
Gel Chromatography
Epithelium
Outcome Assessment (Health Care)

Keywords

  • innate immune defence
  • multimerization
  • single nucleotide polymorphism (SNP)
  • spondyloarthritis
  • surfactant protein-D (SP-D)
  • Pulmonary Surfactant-Associated Protein D/blood
  • Spondylarthritis/blood
  • Humans
  • Middle Aged
  • Protein Multimerization
  • Genotype
  • Male
  • Case-Control Studies
  • Young Adult
  • Inflammation Mediators/blood
  • HLA-B27 Antigen
  • Adult
  • Female
  • Polymorphism, Single Nucleotide

Cite this

@article{d80146e5cc7a4d95af9d7e20230a990b,
title = "Surfactant protein-D, a potential mediator of inflammation in axial spondyloarthritis",
abstract = "Objectives: Surfactant protein-D (SP-D), an innate immune defence molecule of the collectin family, is expressed in lungs and additional extrapulmonary epithelia. SP-D has immune modulatory and anti-microbial effects depending on its oligomerization. The ratio of high molecular weight (HMW): low molecular weight (LMW) SP-D in serum is mainly determined by the Met11Thr polymorphism (SNP rs721917). We aimed to study the SP-D serum level and the molecular size distribution in patients with untreated axial spondyloarthritis (axSpA) as compared with control subjects.Methods: Thirty-four patients with disease modifier untreated axSpA according to the ASAS criteria, age 19-63 years, disease duration 3.9 (2.2-5.6) years were included. Demographics, smoking habits, HLA-B27 status, ASDAS, BASDAI, BASFI, BASMI and visual analogue scale scores were recorded. SP-D in serum was measured by ELISA. DNA was isolated from whole blood and single nucleotide polymorphism rs721917 was genotyped. SP-D molecular size distribution was determined using gel filtration chromatography.Results: SP-D in serum did not differ between patients with axSpA and healthy controls, 1177 (869, 1536) vs 910 (494, 1682) (P = 0.35) and SP-D did not correlate with disease activity. However, the HMW/LMW ratio of SP-D in serum was significantly lower in axSpA, 0.38 (0.18, 0.53) compared with controls 1.49 (0.37, 3.24) when adjusting for the Met11Thr polymorphism, gender, age, BMI and smoking (P = 0.0004). There was no correlation between HMW/LMW ratio and CRP or composite diseases outcome measures.Conclusion: We suggest that predominance of LMW oligomeric variants of SP-D may enhance local or systemic inflammatory responses in axSpA.",
keywords = "innate immune defence, multimerization, single nucleotide polymorphism (SNP), spondyloarthritis, surfactant protein-D (SP-D), Pulmonary Surfactant-Associated Protein D/blood, Spondylarthritis/blood, Humans, Middle Aged, Protein Multimerization, Genotype, Male, Case-Control Studies, Young Adult, Inflammation Mediators/blood, HLA-B27 Antigen, Adult, Female, Polymorphism, Single Nucleotide",
author = "Munk, {Heidi L} and Dalia Fakih and Lene Christiansen and Qihua Tan and Christensen, {Anne F} and Leif Ejstrup and Loft, {Anne G} and Kirsten Junker and Kyvik, {Kirsten O} and Rania Jounblat and Uffe Holmskov and Sorensen, {Grith L} and Peter Junker",
year = "2018",
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doi = "10.1093/rheumatology/key187",
language = "English",
volume = "57",
pages = "1861–1865",
journal = "Rheumatology",
issn = "1462-0324",
publisher = "Heinemann",
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}

Surfactant protein-D, a potential mediator of inflammation in axial spondyloarthritis. / Munk, Heidi L; Fakih, Dalia; Christiansen, Lene; Tan, Qihua; Christensen, Anne F; Ejstrup, Leif; Loft, Anne G; Junker, Kirsten; Kyvik, Kirsten O; Jounblat, Rania; Holmskov, Uffe; Sorensen, Grith L; Junker, Peter.

In: Rheumatology (Oxford, England), Vol. 57, No. 10, 01.10.2018, p. 1861–1865.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Surfactant protein-D, a potential mediator of inflammation in axial spondyloarthritis

AU - Munk, Heidi L

AU - Fakih, Dalia

AU - Christiansen, Lene

AU - Tan, Qihua

AU - Christensen, Anne F

AU - Ejstrup, Leif

AU - Loft, Anne G

AU - Junker, Kirsten

AU - Kyvik, Kirsten O

AU - Jounblat, Rania

AU - Holmskov, Uffe

AU - Sorensen, Grith L

AU - Junker, Peter

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Objectives: Surfactant protein-D (SP-D), an innate immune defence molecule of the collectin family, is expressed in lungs and additional extrapulmonary epithelia. SP-D has immune modulatory and anti-microbial effects depending on its oligomerization. The ratio of high molecular weight (HMW): low molecular weight (LMW) SP-D in serum is mainly determined by the Met11Thr polymorphism (SNP rs721917). We aimed to study the SP-D serum level and the molecular size distribution in patients with untreated axial spondyloarthritis (axSpA) as compared with control subjects.Methods: Thirty-four patients with disease modifier untreated axSpA according to the ASAS criteria, age 19-63 years, disease duration 3.9 (2.2-5.6) years were included. Demographics, smoking habits, HLA-B27 status, ASDAS, BASDAI, BASFI, BASMI and visual analogue scale scores were recorded. SP-D in serum was measured by ELISA. DNA was isolated from whole blood and single nucleotide polymorphism rs721917 was genotyped. SP-D molecular size distribution was determined using gel filtration chromatography.Results: SP-D in serum did not differ between patients with axSpA and healthy controls, 1177 (869, 1536) vs 910 (494, 1682) (P = 0.35) and SP-D did not correlate with disease activity. However, the HMW/LMW ratio of SP-D in serum was significantly lower in axSpA, 0.38 (0.18, 0.53) compared with controls 1.49 (0.37, 3.24) when adjusting for the Met11Thr polymorphism, gender, age, BMI and smoking (P = 0.0004). There was no correlation between HMW/LMW ratio and CRP or composite diseases outcome measures.Conclusion: We suggest that predominance of LMW oligomeric variants of SP-D may enhance local or systemic inflammatory responses in axSpA.

AB - Objectives: Surfactant protein-D (SP-D), an innate immune defence molecule of the collectin family, is expressed in lungs and additional extrapulmonary epithelia. SP-D has immune modulatory and anti-microbial effects depending on its oligomerization. The ratio of high molecular weight (HMW): low molecular weight (LMW) SP-D in serum is mainly determined by the Met11Thr polymorphism (SNP rs721917). We aimed to study the SP-D serum level and the molecular size distribution in patients with untreated axial spondyloarthritis (axSpA) as compared with control subjects.Methods: Thirty-four patients with disease modifier untreated axSpA according to the ASAS criteria, age 19-63 years, disease duration 3.9 (2.2-5.6) years were included. Demographics, smoking habits, HLA-B27 status, ASDAS, BASDAI, BASFI, BASMI and visual analogue scale scores were recorded. SP-D in serum was measured by ELISA. DNA was isolated from whole blood and single nucleotide polymorphism rs721917 was genotyped. SP-D molecular size distribution was determined using gel filtration chromatography.Results: SP-D in serum did not differ between patients with axSpA and healthy controls, 1177 (869, 1536) vs 910 (494, 1682) (P = 0.35) and SP-D did not correlate with disease activity. However, the HMW/LMW ratio of SP-D in serum was significantly lower in axSpA, 0.38 (0.18, 0.53) compared with controls 1.49 (0.37, 3.24) when adjusting for the Met11Thr polymorphism, gender, age, BMI and smoking (P = 0.0004). There was no correlation between HMW/LMW ratio and CRP or composite diseases outcome measures.Conclusion: We suggest that predominance of LMW oligomeric variants of SP-D may enhance local or systemic inflammatory responses in axSpA.

KW - innate immune defence

KW - multimerization

KW - single nucleotide polymorphism (SNP)

KW - spondyloarthritis

KW - surfactant protein-D (SP-D)

KW - Pulmonary Surfactant-Associated Protein D/blood

KW - Spondylarthritis/blood

KW - Humans

KW - Middle Aged

KW - Protein Multimerization

KW - Genotype

KW - Male

KW - Case-Control Studies

KW - Young Adult

KW - Inflammation Mediators/blood

KW - HLA-B27 Antigen

KW - Adult

KW - Female

KW - Polymorphism, Single Nucleotide

U2 - 10.1093/rheumatology/key187

DO - 10.1093/rheumatology/key187

M3 - Journal article

C2 - 29982797

VL - 57

SP - 1861

EP - 1865

JO - Rheumatology

JF - Rheumatology

SN - 1462-0324

IS - 10

ER -