Sub-diffraction positioning of a two-photon excited and optically trapped quantum dot

L. Jauffred, A. Kyrsting, Eva Arnspang Christensen, S. N. S. Reihani, L. B. Oddershede

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Colloidal quantum dots are luminescent long-lived probes that can be two-photon excited and manipulated by a single laser beam. Therefore, quantum dots can be used for simultaneous single molecule visualization and force manipulation using an infra-red laser. Here, we show that even a single optically trapped quantum dot, performing restricted Brownian motion within the focal volume, can be two-photon excited by the trapping laser beam and its luminescence can be detected by a camera. After two-photon excitation for a time long enough, the emitted light from the quantum dot is shown to blueshift. A quantum dot is much smaller than a diffraction limited laser focus and by mapping out the intensity of the focal volume and overlaying this with the positions visited by a quantum dot, a quantum dot is shown often to explore regions of the focal volume where the intensity is too low to render two-photon absorption likely. This is in accordance with the observation that a trapped quantum dot is only fluorescing 5-10 percent of the time. The results are important for realizing nano-scale quantum dot control and visualization and for correct interpretation of experiments using two-photon excited quantum dots as markers.
Original languageEnglish
JournalNanoscale
Volume6
Issue number12
Pages (from-to)6997-7003
ISSN2040-3364
DOIs
Publication statusPublished - 2014

Cite this

Jauffred, L. ; Kyrsting, A. ; Christensen, Eva Arnspang ; Reihani, S. N. S. ; Oddershede, L. B. / Sub-diffraction positioning of a two-photon excited and optically trapped quantum dot. In: Nanoscale. 2014 ; Vol. 6, No. 12. pp. 6997-7003.
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abstract = "Colloidal quantum dots are luminescent long-lived probes that can be two-photon excited and manipulated by a single laser beam. Therefore, quantum dots can be used for simultaneous single molecule visualization and force manipulation using an infra-red laser. Here, we show that even a single optically trapped quantum dot, performing restricted Brownian motion within the focal volume, can be two-photon excited by the trapping laser beam and its luminescence can be detected by a camera. After two-photon excitation for a time long enough, the emitted light from the quantum dot is shown to blueshift. A quantum dot is much smaller than a diffraction limited laser focus and by mapping out the intensity of the focal volume and overlaying this with the positions visited by a quantum dot, a quantum dot is shown often to explore regions of the focal volume where the intensity is too low to render two-photon absorption likely. This is in accordance with the observation that a trapped quantum dot is only fluorescing 5-10 percent of the time. The results are important for realizing nano-scale quantum dot control and visualization and for correct interpretation of experiments using two-photon excited quantum dots as markers.",
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author = "L. Jauffred and A. Kyrsting and Christensen, {Eva Arnspang} and Reihani, {S. N. S.} and Oddershede, {L. B.}",
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Jauffred, L, Kyrsting, A, Christensen, EA, Reihani, SNS & Oddershede, LB 2014, 'Sub-diffraction positioning of a two-photon excited and optically trapped quantum dot', Nanoscale, vol. 6, no. 12, pp. 6997-7003. https://doi.org/10.1039/c4nr01319k

Sub-diffraction positioning of a two-photon excited and optically trapped quantum dot. / Jauffred, L.; Kyrsting, A.; Christensen, Eva Arnspang; Reihani, S. N. S.; Oddershede, L. B.

In: Nanoscale, Vol. 6, No. 12, 2014, p. 6997-7003.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Sub-diffraction positioning of a two-photon excited and optically trapped quantum dot

AU - Jauffred, L.

AU - Kyrsting, A.

AU - Christensen, Eva Arnspang

AU - Reihani, S. N. S.

AU - Oddershede, L. B.

N1 - ISI Document Delivery No.: AI8FI Times Cited: 0 Cited Reference Count: 38 Jauffred, Liselotte Kyrsting, Anders Arnspang, Eva C. Reihani, S. Nader S. Oddershede, Lene B. Carlsberg Foundation; Lundbeck Foundation; University of Copenhagen Excellence Program We thank C. B. Lagerholm and MEMPHYS for lending us the QuadView image splitter and H. Ma for experimental assistance. We acknowledge financial support from the Carlsberg Foundation, the Lundbeck Foundation, and from the University of Copenhagen Excellence Program. 0 ROYAL SOC CHEMISTRY CAMBRIDGE NANOSCALE

PY - 2014

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N2 - Colloidal quantum dots are luminescent long-lived probes that can be two-photon excited and manipulated by a single laser beam. Therefore, quantum dots can be used for simultaneous single molecule visualization and force manipulation using an infra-red laser. Here, we show that even a single optically trapped quantum dot, performing restricted Brownian motion within the focal volume, can be two-photon excited by the trapping laser beam and its luminescence can be detected by a camera. After two-photon excitation for a time long enough, the emitted light from the quantum dot is shown to blueshift. A quantum dot is much smaller than a diffraction limited laser focus and by mapping out the intensity of the focal volume and overlaying this with the positions visited by a quantum dot, a quantum dot is shown often to explore regions of the focal volume where the intensity is too low to render two-photon absorption likely. This is in accordance with the observation that a trapped quantum dot is only fluorescing 5-10 percent of the time. The results are important for realizing nano-scale quantum dot control and visualization and for correct interpretation of experiments using two-photon excited quantum dots as markers.

AB - Colloidal quantum dots are luminescent long-lived probes that can be two-photon excited and manipulated by a single laser beam. Therefore, quantum dots can be used for simultaneous single molecule visualization and force manipulation using an infra-red laser. Here, we show that even a single optically trapped quantum dot, performing restricted Brownian motion within the focal volume, can be two-photon excited by the trapping laser beam and its luminescence can be detected by a camera. After two-photon excitation for a time long enough, the emitted light from the quantum dot is shown to blueshift. A quantum dot is much smaller than a diffraction limited laser focus and by mapping out the intensity of the focal volume and overlaying this with the positions visited by a quantum dot, a quantum dot is shown often to explore regions of the focal volume where the intensity is too low to render two-photon absorption likely. This is in accordance with the observation that a trapped quantum dot is only fluorescing 5-10 percent of the time. The results are important for realizing nano-scale quantum dot control and visualization and for correct interpretation of experiments using two-photon excited quantum dots as markers.

KW - DIELECTRIC INTERFACE ELECTRIC-DIPOLES LIGHT-EMISSION NANOPARTICLES ORIENTATION TWEEZERS TRACKING CELLS

U2 - 10.1039/c4nr01319k

DO - 10.1039/c4nr01319k

M3 - Journal article

C2 - 24839080

VL - 6

SP - 6997

EP - 7003

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 12

ER -