SPR analysis of antibody-antigen interactions using graphene oxide linking layers

Yu V. Stebunov; Aleksey V.  Arsenin; Valentyn S. Volkov

doi:10.1016/j.matpr.2018.06.047

SPR analysis of antibody-antigen interactions using graphene oxide linking layers

Yu V. Stebunov, Aleksey V. Arsenin, Valentyn S. Volkov

Research output: Contribution to journal › Conference article › Research › peer-review

Abstract

Surface plasmon resonance (SPR) biosensing provides a powerful tool for label-free kinetic analysis of antibody-antigen interactions. SPR analysis can be improved with biosensing interfaces based on graphene oxide (GO), which will enhance the accuracy of determination of kinetic constants and expand a range of suitable targets. GO possesses large surface area and oxygen-containing functional groups for covalent immobilization of biomolecules. Here, we are proposing an SPR biosensing interface based on a GO linking layer with immobilized protein A, which allows orientation-specific immobilization of antibodies. Protein A was covalently attached to the surface of GO via amine coupling. Thereafter, the developed biosensing interface was used for the analysis of the interaction between anti-bovine serum albumin (BSA) antibody and BSA.

Original language	English
Journal	Materials Today: Proceedings
Volume	5
Issue number	9 (Part 2)
Pages (from-to)	17442-17446
ISSN	2214-7853
DOIs	https://doi.org/10.1016/j.matpr.2018.06.047
Publication status	Published - 2018
Event	9th International Conference on Advanced Nano Materials - University of Aveiro, Portugal Duration: 19. Jul 2017 → 21. Jul 2017

Conference

Conference	9th International Conference on Advanced Nano Materials
Location	University of Aveiro
Country/Territory	Portugal
Period	19/07/2017 → 21/07/2017

Keywords

Antibody-antigen interaction
Biosensing interfaces
Graphene oxide
Protein A
Surface plasmon resonance

Documents & Links

10.1016/j.matpr.2018.06.047

Cite this

@inproceedings{91543f99c5ab4d7fb1f0c0cf38404746,

title = "SPR analysis of antibody-antigen interactions using graphene oxide linking layers",

abstract = "Surface plasmon resonance (SPR) biosensing provides a powerful tool for label-free kinetic analysis of antibody-antigen interactions. SPR analysis can be improved with biosensing interfaces based on graphene oxide (GO), which will enhance the accuracy of determination of kinetic constants and expand a range of suitable targets. GO possesses large surface area and oxygen-containing functional groups for covalent immobilization of biomolecules. Here, we are proposing an SPR biosensing interface based on a GO linking layer with immobilized protein A, which allows orientation-specific immobilization of antibodies. Protein A was covalently attached to the surface of GO via amine coupling. Thereafter, the developed biosensing interface was used for the analysis of the interaction between anti-bovine serum albumin (BSA) antibody and BSA.",

keywords = "Antibody-antigen interaction, Biosensing interfaces, Graphene oxide, Protein A, Surface plasmon resonance",

author = "Stebunov, {Yu V.} and Arsenin, {Aleksey V.} and Volkov, {Valentyn S.}",

year = "2018",

doi = "10.1016/j.matpr.2018.06.047",

language = "English",

volume = "5",

pages = "17442--17446",

journal = "Materials Today: Proceedings",

issn = "2214-7853",

publisher = "Elsevier",

number = "9 (Part 2)",

note = "9th International Conference on Advanced Nano Materials, ANM 2017 ; Conference date: 19-07-2017 Through 21-07-2017",

}

TY - GEN

T1 - SPR analysis of antibody-antigen interactions using graphene oxide linking layers

AU - Stebunov, Yu V.

AU - Arsenin, Aleksey V.

AU - Volkov, Valentyn S.

PY - 2018

Y1 - 2018

N2 - Surface plasmon resonance (SPR) biosensing provides a powerful tool for label-free kinetic analysis of antibody-antigen interactions. SPR analysis can be improved with biosensing interfaces based on graphene oxide (GO), which will enhance the accuracy of determination of kinetic constants and expand a range of suitable targets. GO possesses large surface area and oxygen-containing functional groups for covalent immobilization of biomolecules. Here, we are proposing an SPR biosensing interface based on a GO linking layer with immobilized protein A, which allows orientation-specific immobilization of antibodies. Protein A was covalently attached to the surface of GO via amine coupling. Thereafter, the developed biosensing interface was used for the analysis of the interaction between anti-bovine serum albumin (BSA) antibody and BSA.

AB - Surface plasmon resonance (SPR) biosensing provides a powerful tool for label-free kinetic analysis of antibody-antigen interactions. SPR analysis can be improved with biosensing interfaces based on graphene oxide (GO), which will enhance the accuracy of determination of kinetic constants and expand a range of suitable targets. GO possesses large surface area and oxygen-containing functional groups for covalent immobilization of biomolecules. Here, we are proposing an SPR biosensing interface based on a GO linking layer with immobilized protein A, which allows orientation-specific immobilization of antibodies. Protein A was covalently attached to the surface of GO via amine coupling. Thereafter, the developed biosensing interface was used for the analysis of the interaction between anti-bovine serum albumin (BSA) antibody and BSA.

KW - Antibody-antigen interaction

KW - Biosensing interfaces

KW - Graphene oxide

KW - Protein A

KW - Surface plasmon resonance

U2 - 10.1016/j.matpr.2018.06.047

DO - 10.1016/j.matpr.2018.06.047

M3 - Conference article

SN - 2214-7853

VL - 5

SP - 17442

EP - 17446

JO - Materials Today: Proceedings

JF - Materials Today: Proceedings

IS - 9 (Part 2)

T2 - 9th International Conference on Advanced Nano Materials

Y2 - 19 July 2017 through 21 July 2017

ER -

SPR analysis of antibody-antigen interactions using graphene oxide linking layers

Abstract

Conference

Keywords

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