Spatial and Temporal Mapping of Human Innate Lymphoid Cells Reveals Elements of Tissue Specificity

Naomi A Yudanin, Frederike Schmitz, Anne-Laure Flamar, Joseph J C Thome, Elia Tait Wojno, Jesper B Moeller, Melanie Schirmer, Isabel J Latorre, Ramnik J Xavier, Donna L Farber, Laurel A Monticelli, David Artis

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Innate lymphoid cells (ILC) play critical roles in regulating immunity, inflammation, and tissue homeostasis in mice. However, limited access to non-diseased human tissues has hindered efforts to profile anatomically-distinct ILCs in humans. Through flow cytometric and transcriptional analyses of lymphoid, mucosal, and metabolic tissues from previously healthy human organ donors, here we have provided a map of human ILC heterogeneity across multiple anatomical sites. In contrast to mice, human ILCs are less strictly compartmentalized and tissue localization selectively impacts ILC distribution in a subset-dependent manner. Tissue-specific distinctions are particularly apparent for ILC1 populations, whose distribution was markedly altered in obesity or aging. Furthermore, the degree of ILC1 population heterogeneity differed substantially in lymphoid versus mucosal sites. Together, these analyses comprise a comprehensive characterization of the spatial and temporal dynamics regulating the anatomical distribution, subset heterogeneity, and functional potential of ILCs in non-diseased human tissues.

Original languageEnglish
JournalImmunity
Volume50
Issue number2
Pages (from-to)505-519.e4
ISSN1074-7613
DOIs
Publication statusPublished - 19. Feb 2019
Externally publishedYes

Keywords

  • Adolescent
  • Adult
  • Aged
  • Aging/genetics
  • Animals
  • Child
  • Child, Preschool
  • Female
  • Genetic Heterogeneity
  • Humans
  • Immunity, Innate/genetics
  • Infant
  • Infant, Newborn
  • Lymphocytes/immunology
  • Male
  • Mice
  • Middle Aged
  • Organ Specificity/genetics
  • Transcriptome/genetics
  • Young Adult

Fingerprint Dive into the research topics of 'Spatial and Temporal Mapping of Human Innate Lymphoid Cells Reveals Elements of Tissue Specificity'. Together they form a unique fingerprint.

  • Cite this

    Yudanin, N. A., Schmitz, F., Flamar, A-L., Thome, J. J. C., Tait Wojno, E., Moeller, J. B., Schirmer, M., Latorre, I. J., Xavier, R. J., Farber, D. L., Monticelli, L. A., & Artis, D. (2019). Spatial and Temporal Mapping of Human Innate Lymphoid Cells Reveals Elements of Tissue Specificity. Immunity, 50(2), 505-519.e4. https://doi.org/10.1016/j.immuni.2019.01.012