Single Molecule Applications of Quantum Dots

Thomas Elmelund Rasmussen, Liselotte Jauffred, Jonathan R. Brewer, Stefan Vogel, Esben Rossel Torbensen, B. Christoffer Lagerholm, Lene Oddershede, Eva Arnspang Christensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Fluorescent nanocrystals composed of semiconductor materials were first introduced for biological applications in the late 1990s. The focus of this review is to give a brief survey of biological applications of quantum dots (QDs) at the single QD sensitivity level. These are described as follows: 1) QD blinking and bleaching statistics, 2) the use of QDs in high speed single particle tracking with a special focus on how to design the biofunctional coatings of QDs which enable specific targeting to single proteins or lipids of interest, 3) a hybrid lipid-DNA analogue binding QDs which allows for tracking single lipids in lipid bilayers, 4) two-photon fluorescence correlation spectroscopy of QDs and 5) optical trapping and excitation of single QDs. In all of these applications, the focus is on the single particle sensitivity level of QDs. The high applicability of QDs in live cell imaging experiments held together with the prospects in localization microscopy and single molecule manipulation experiments gave QDs a promising future in single molecule research.
Original languageEnglish
JournalJournal of Modern Physics
Volume4
Pages (from-to)27-42
ISSN2153-1196
DOIs
Publication statusPublished - 2013

Fingerprint

Semiconductor quantum dots
Molecules
Lipids
Lipid bilayers
Bleaching
Nanocrystals
Microscopic examination
Photons
Experiments
Fluorescence
Statistics
Spectroscopy
Semiconductor materials
Imaging techniques
Coatings
DNA

Keywords

  • Quantum Dots, microscopy

Cite this

Rasmussen, Thomas Elmelund ; Jauffred, Liselotte ; Brewer, Jonathan R. ; Vogel, Stefan ; Torbensen, Esben Rossel ; Lagerholm, B. Christoffer ; Oddershede, Lene ; Christensen, Eva Arnspang. / Single Molecule Applications of Quantum Dots. In: Journal of Modern Physics. 2013 ; Vol. 4. pp. 27-42.
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abstract = "Fluorescent nanocrystals composed of semiconductor materials were first introduced for biological applications in the late 1990s. The focus of this review is to give a brief survey of biological applications of quantum dots (QDs) at the single QD sensitivity level. These are described as follows: 1) QD blinking and bleaching statistics, 2) the use of QDs in high speed single particle tracking with a special focus on how to design the biofunctional coatings of QDs which enable specific targeting to single proteins or lipids of interest, 3) a hybrid lipid-DNA analogue binding QDs which allows for tracking single lipids in lipid bilayers, 4) two-photon fluorescence correlation spectroscopy of QDs and 5) optical trapping and excitation of single QDs. In all of these applications, the focus is on the single particle sensitivity level of QDs. The high applicability of QDs in live cell imaging experiments held together with the prospects in localization microscopy and single molecule manipulation experiments gave QDs a promising future in single molecule research.",
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Rasmussen, TE, Jauffred, L, Brewer, JR, Vogel, S, Torbensen, ER, Lagerholm, BC, Oddershede, L & Christensen, EA 2013, 'Single Molecule Applications of Quantum Dots', Journal of Modern Physics, vol. 4, pp. 27-42. https://doi.org/10.4236/jmp.2013.411B002

Single Molecule Applications of Quantum Dots. / Rasmussen, Thomas Elmelund; Jauffred, Liselotte ; Brewer, Jonathan R.; Vogel, Stefan; Torbensen, Esben Rossel; Lagerholm, B. Christoffer; Oddershede, Lene; Christensen, Eva Arnspang.

In: Journal of Modern Physics, Vol. 4, 2013, p. 27-42.

Research output: Contribution to journalJournal articleResearchpeer-review

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AU - Rasmussen, Thomas Elmelund

AU - Jauffred, Liselotte

AU - Brewer, Jonathan R.

AU - Vogel, Stefan

AU - Torbensen, Esben Rossel

AU - Lagerholm, B. Christoffer

AU - Oddershede, Lene

AU - Christensen, Eva Arnspang

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