Simultaneous Multi-Species Tracking in Live Cells with Quantum Dot Conjugates

M. P. Clausen, Eva Arnspang Christensen, B. Ballou, J. E. Bear, B. C. Lagerholm

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Quantum dots are available in a range of spectrally separated emission colors and with a range of water-stabilizing surface coatings that offers great flexibility for enabling bio-specificity. In this study, we have taken advantage of this flexibility to demonstrate that it is possible to perform a simultaneous investigation of the lateral dynamics in the plasma membrane of i) the transmembrane epidermal growth factor receptor, ii) the glucosylphospatidylinositol-anchored protein CD59, and iii) ganglioside G(M1)-cholera toxin subunit B clusters in a single cell. We show that a large number of the trajectories are longer than 50 steps, which we by simulations show to be sufficient for robust single trajectory analysis. This analysis shows that the populations of the diffusion coefficients are heterogeneously distributed for all three species, but differ between the different species. We further show that the heterogeneity is decreased upon treating the cells with methyl-beta-cyclodextrin.
Original languageEnglish
Article numbere97671
JournalP L o S One
Volume9
Issue number6
Number of pages12
ISSN1932-6203
DOIs
Publication statusPublished - 2014

Cite this

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title = "Simultaneous Multi-Species Tracking in Live Cells with Quantum Dot Conjugates",
abstract = "Quantum dots are available in a range of spectrally separated emission colors and with a range of water-stabilizing surface coatings that offers great flexibility for enabling bio-specificity. In this study, we have taken advantage of this flexibility to demonstrate that it is possible to perform a simultaneous investigation of the lateral dynamics in the plasma membrane of i) the transmembrane epidermal growth factor receptor, ii) the glucosylphospatidylinositol-anchored protein CD59, and iii) ganglioside G(M1)-cholera toxin subunit B clusters in a single cell. We show that a large number of the trajectories are longer than 50 steps, which we by simulations show to be sufficient for robust single trajectory analysis. This analysis shows that the populations of the diffusion coefficients are heterogeneously distributed for all three species, but differ between the different species. We further show that the heterogeneity is decreased upon treating the cells with methyl-beta-cyclodextrin.",
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Simultaneous Multi-Species Tracking in Live Cells with Quantum Dot Conjugates. / Clausen, M. P.; Christensen, Eva Arnspang; Ballou, B.; Bear, J. E.; Lagerholm, B. C.

In: P L o S One, Vol. 9, No. 6, e97671, 2014.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Simultaneous Multi-Species Tracking in Live Cells with Quantum Dot Conjugates

AU - Clausen, M. P.

AU - Christensen, Eva Arnspang

AU - Ballou, B.

AU - Bear, J. E.

AU - Lagerholm, B. C.

PY - 2014

Y1 - 2014

N2 - Quantum dots are available in a range of spectrally separated emission colors and with a range of water-stabilizing surface coatings that offers great flexibility for enabling bio-specificity. In this study, we have taken advantage of this flexibility to demonstrate that it is possible to perform a simultaneous investigation of the lateral dynamics in the plasma membrane of i) the transmembrane epidermal growth factor receptor, ii) the glucosylphospatidylinositol-anchored protein CD59, and iii) ganglioside G(M1)-cholera toxin subunit B clusters in a single cell. We show that a large number of the trajectories are longer than 50 steps, which we by simulations show to be sufficient for robust single trajectory analysis. This analysis shows that the populations of the diffusion coefficients are heterogeneously distributed for all three species, but differ between the different species. We further show that the heterogeneity is decreased upon treating the cells with methyl-beta-cyclodextrin.

AB - Quantum dots are available in a range of spectrally separated emission colors and with a range of water-stabilizing surface coatings that offers great flexibility for enabling bio-specificity. In this study, we have taken advantage of this flexibility to demonstrate that it is possible to perform a simultaneous investigation of the lateral dynamics in the plasma membrane of i) the transmembrane epidermal growth factor receptor, ii) the glucosylphospatidylinositol-anchored protein CD59, and iii) ganglioside G(M1)-cholera toxin subunit B clusters in a single cell. We show that a large number of the trajectories are longer than 50 steps, which we by simulations show to be sufficient for robust single trajectory analysis. This analysis shows that the populations of the diffusion coefficients are heterogeneously distributed for all three species, but differ between the different species. We further show that the heterogeneity is decreased upon treating the cells with methyl-beta-cyclodextrin.

U2 - 10.1371/journal.pone.0097671

DO - 10.1371/journal.pone.0097671

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