Shifts in microbiome structure during summer stratification in temperate lakes

Kamilla Schneekloth Sjøgaard, Michael Forth, Esther Singer, Andreas Schramm, Tanja Woyke, Alexander H. Treusch

Research output: Contribution to conference without publisher/journalPosterResearchpeer-review

Abstract

Temperate lakes often feature a seasonal stratification of the water column, resulting in a hypoxic or anoxic hypolimnion. This heavily impacts the microbiome, however, we only have started to understand how this perturbation effects the ecological connections between different microbes and their abilities for the biogeochemical cycling of elements. Here we present the spatiotemporal analysis of the microbiomes of two meso-/oligotrophic dimictic lakes experiencing summer stratification. By employing multivariate statistics and cooccurrence network analyses of 16S rRNA gene amplicon data, we observed the clustering of bacterial communities correlated to oxygen concentrations throughout stratification. Cooccurrence patterns among abundant and ubiquitous freshwater Actinobacteria and Proteobacteria suggested microbial niche partitioning potentially accompanied by differential specialization in carbon substrate metabolisms. The hypolimnion showed diverse communities with the potential for a wide variety of respiration processes. Hypoxic/anoxic
conditions distinctly defined a module of OTUs co-occurring in the hypolimnion during summer stratification, containing anaerobic Chloroflexi and Chlorobi, but also methanotrophs. We further detected a module including OTUs of the candidate division OP3, Planctomycetes and Nitrosomonadaceae, occurring at low temperatures in the hypolimnion, predominantly in the oxygen gradient before complete anoxic conditions developed.
Original languageEnglish
Publication date12. Nov 2018
Publication statusPublished - 12. Nov 2018
EventThe Danish Microbiological Society - Annual Congress 2018 - Eigtveds Pakhus, København, Denmark
Duration: 12. Nov 201812. Nov 2018

Conference

ConferenceThe Danish Microbiological Society - Annual Congress 2018
LocationEigtveds Pakhus
CountryDenmark
CityKøbenhavn
Period12/11/201812/11/2018

Fingerprint

hypolimnion
stratification
lake
summer
spatiotemporal analysis
oxygen
niche partitioning
anoxic conditions
respiration
water column
metabolism
perturbation
substrate
gene
carbon

Cite this

Sjøgaard, K. S., Forth, M., Singer, E., Schramm, A., Woyke, T., & Treusch, A. H. (2018). Shifts in microbiome structure during summer stratification in temperate lakes. Poster session presented at The Danish Microbiological Society - Annual Congress 2018, København, Denmark.
Sjøgaard, Kamilla Schneekloth ; Forth, Michael ; Singer, Esther ; Schramm, Andreas ; Woyke, Tanja ; Treusch, Alexander H. / Shifts in microbiome structure during summer stratification in temperate lakes. Poster session presented at The Danish Microbiological Society - Annual Congress 2018, København, Denmark.
@conference{bed7e3eee21f4a3c9a97b26fc32faf3e,
title = "Shifts in microbiome structure during summer stratification in temperate lakes",
abstract = "Temperate lakes often feature a seasonal stratification of the water column, resulting in a hypoxic or anoxic hypolimnion. This heavily impacts the microbiome, however, we only have started to understand how this perturbation effects the ecological connections between different microbes and their abilities for the biogeochemical cycling of elements. Here we present the spatiotemporal analysis of the microbiomes of two meso-/oligotrophic dimictic lakes experiencing summer stratification. By employing multivariate statistics and cooccurrence network analyses of 16S rRNA gene amplicon data, we observed the clustering of bacterial communities correlated to oxygen concentrations throughout stratification. Cooccurrence patterns among abundant and ubiquitous freshwater Actinobacteria and Proteobacteria suggested microbial niche partitioning potentially accompanied by differential specialization in carbon substrate metabolisms. The hypolimnion showed diverse communities with the potential for a wide variety of respiration processes. Hypoxic/anoxicconditions distinctly defined a module of OTUs co-occurring in the hypolimnion during summer stratification, containing anaerobic Chloroflexi and Chlorobi, but also methanotrophs. We further detected a module including OTUs of the candidate division OP3, Planctomycetes and Nitrosomonadaceae, occurring at low temperatures in the hypolimnion, predominantly in the oxygen gradient before complete anoxic conditions developed.",
author = "Sj{\o}gaard, {Kamilla Schneekloth} and Michael Forth and Esther Singer and Andreas Schramm and Tanja Woyke and Treusch, {Alexander H.}",
year = "2018",
month = "11",
day = "12",
language = "English",
note = "null ; Conference date: 12-11-2018 Through 12-11-2018",

}

Sjøgaard, KS, Forth, M, Singer, E, Schramm, A, Woyke, T & Treusch, AH 2018, 'Shifts in microbiome structure during summer stratification in temperate lakes', The Danish Microbiological Society - Annual Congress 2018, København, Denmark, 12/11/2018 - 12/11/2018.

Shifts in microbiome structure during summer stratification in temperate lakes. / Sjøgaard, Kamilla Schneekloth; Forth, Michael; Singer, Esther; Schramm, Andreas; Woyke, Tanja; Treusch, Alexander H.

2018. Poster session presented at The Danish Microbiological Society - Annual Congress 2018, København, Denmark.

Research output: Contribution to conference without publisher/journalPosterResearchpeer-review

TY - CONF

T1 - Shifts in microbiome structure during summer stratification in temperate lakes

AU - Sjøgaard, Kamilla Schneekloth

AU - Forth, Michael

AU - Singer, Esther

AU - Schramm, Andreas

AU - Woyke, Tanja

AU - Treusch, Alexander H.

PY - 2018/11/12

Y1 - 2018/11/12

N2 - Temperate lakes often feature a seasonal stratification of the water column, resulting in a hypoxic or anoxic hypolimnion. This heavily impacts the microbiome, however, we only have started to understand how this perturbation effects the ecological connections between different microbes and their abilities for the biogeochemical cycling of elements. Here we present the spatiotemporal analysis of the microbiomes of two meso-/oligotrophic dimictic lakes experiencing summer stratification. By employing multivariate statistics and cooccurrence network analyses of 16S rRNA gene amplicon data, we observed the clustering of bacterial communities correlated to oxygen concentrations throughout stratification. Cooccurrence patterns among abundant and ubiquitous freshwater Actinobacteria and Proteobacteria suggested microbial niche partitioning potentially accompanied by differential specialization in carbon substrate metabolisms. The hypolimnion showed diverse communities with the potential for a wide variety of respiration processes. Hypoxic/anoxicconditions distinctly defined a module of OTUs co-occurring in the hypolimnion during summer stratification, containing anaerobic Chloroflexi and Chlorobi, but also methanotrophs. We further detected a module including OTUs of the candidate division OP3, Planctomycetes and Nitrosomonadaceae, occurring at low temperatures in the hypolimnion, predominantly in the oxygen gradient before complete anoxic conditions developed.

AB - Temperate lakes often feature a seasonal stratification of the water column, resulting in a hypoxic or anoxic hypolimnion. This heavily impacts the microbiome, however, we only have started to understand how this perturbation effects the ecological connections between different microbes and their abilities for the biogeochemical cycling of elements. Here we present the spatiotemporal analysis of the microbiomes of two meso-/oligotrophic dimictic lakes experiencing summer stratification. By employing multivariate statistics and cooccurrence network analyses of 16S rRNA gene amplicon data, we observed the clustering of bacterial communities correlated to oxygen concentrations throughout stratification. Cooccurrence patterns among abundant and ubiquitous freshwater Actinobacteria and Proteobacteria suggested microbial niche partitioning potentially accompanied by differential specialization in carbon substrate metabolisms. The hypolimnion showed diverse communities with the potential for a wide variety of respiration processes. Hypoxic/anoxicconditions distinctly defined a module of OTUs co-occurring in the hypolimnion during summer stratification, containing anaerobic Chloroflexi and Chlorobi, but also methanotrophs. We further detected a module including OTUs of the candidate division OP3, Planctomycetes and Nitrosomonadaceae, occurring at low temperatures in the hypolimnion, predominantly in the oxygen gradient before complete anoxic conditions developed.

M3 - Poster

ER -

Sjøgaard KS, Forth M, Singer E, Schramm A, Woyke T, Treusch AH. Shifts in microbiome structure during summer stratification in temperate lakes. 2018. Poster session presented at The Danish Microbiological Society - Annual Congress 2018, København, Denmark.