Serological Assessment of the Quality of Wound Healing Processes in Crohn's Disease

Shu Sun, Morten Asser Karsdal, Joachim Høg Mortensen, Yunyun Luo, Jens Kjeldsen, Aleksander Krag, Michael Dam Jensen, Anne Christine Bay-Jensen, Tina Manon-Jensen

Research output: Contribution to journalJournal articleResearchpeer-review

84 Downloads (Pure)


BACKGROUND AND AIMS: Crohn's disease (CD) is a chronic inflammatory condition characterized by continuous mucosal damage and ongoing wound healing of the intestines. The fibrinolytic system is involved in early parts of the wound healing process. Fibrin is a key mediator of primary blood clot formation and is formed by cross-linking of fibrinogen. To gain insights into the dynamics of wound healing in CD patients we investigated the conversion of fibrinogen into fibrin by the pro-peptide FPA, the amount of factor XIII cross-linked fibrin and total fibrin clot. METHODS: Serum samples of 35 CD patients, 15 non-inflammatory bowel disease (non-IBD) patients and 39 age-matched healthy controls were analyzed for three novel neo-epitope markers: D-fragment and D-dimer, reflecting the degradation of total fibrin clot and factor XIII cross-linked fibrin, as well as FPA, reflecting synthesis of fibrin. RESULTS: Crohn's disease patients had a significantly lower D-dimer level (p=0.0001) compared to healthy controls. Crohn's disease and non-IBD patients had a significantly higher level of FPA (p<0.0001) and D-fragment/D-dimer ratio (p<0.0001 and p=0.02). FPA, D-dimer and D-fragment/D-dimer ratio could distinguish CD patients from healthy controls with area under the curve of 0.92 (95% CI 0.83-0.97), 0.78 (95% CI 0.67-0.87) and 0.85 (95% CI 0.75-0.93), respectively. CONCLUSION: Wound healing parameters were clearly changed in CD patients. FPA levels were higher in CD patients as compared to healthy controls, indicating more ongoing wound healing. D-dimer levels were lower in CD patients than in healthy controls, indicating impaired wound healing due to poor quality of factor XIII cross-linked fibrin and clot resolution.

Original languageEnglish
JournalJournal of gastrointestinal and liver diseases : JGLD
Issue number2
Pages (from-to)175-182
Publication statusPublished - 1. Jun 2019


  • Crohn’s disease
  • D-dimer
  • Fibrin
  • Fibrinogen
  • Fibrinopeptide A


Dive into the research topics of 'Serological Assessment of the Quality of Wound Healing Processes in Crohn's Disease'. Together they form a unique fingerprint.

Cite this