Sequencing of bacteria or other species

David A. Weitz; Huidan Zhang; Nai Wen CUI; Yamei Cai; Peter Girguis; Frank Stewart; Neha Sarode; Beate Kraft

Sequencing of bacteria or other species

David A. Weitz (Inventor)
, Huidan Zhang (Inventor)
, Nai Wen CUI (Inventor)
, Yamei Cai (Inventor)
, Peter Girguis (Inventor)
, Frank Stewart (Inventor)
, Neha Sarode (Inventor)
, Beate Kraft (Inventor)

Harvard University
Georgia Institute of Technology

Research output: Patent

Abstract

The present invention generally relates to sequencing of cells such as bacteria or archeons. In one aspect, cells are lysed to release RNA, which is then polyadenylated and exposed to a sequencing having an identification portion and a poly-T portion, which associates with the RNA. These are then reverse transcribed to produce cDNA, which can then be studied, amplified, etc. In some cases, such reactions may be performed within droplets. The droplets can then be broken to release the cDNAs, which may be combined together for further sequencing, analysis, etc.

Original language	English
IPC	C12N 15/10 (2006.01),C12Q 1/68 (2006 .01)
Patent number	WO 2018/067792 A1
Filing date	05/10/2017
Country/Territory	United States
Priority date	01/07/2016
Priority number	US201662405775P
Publication status	Published - 12. Apr 2018

SDU Link

Check access to the material in SDU Library's search engine

Cite this

@misc{7552f01adfdd4584b5b86be8c9b04d41,

title = "Sequencing of bacteria or other species",

abstract = "The present invention generally relates to sequencing of cells such as bacteria or archeons. In one aspect, cells are lysed to release RNA, which is then polyadenylated and exposed to a sequencing having an identification portion and a poly-T portion, which associates with the RNA. These are then reverse transcribed to produce cDNA, which can then be studied, amplified, etc. In some cases, such reactions may be performed within droplets. The droplets can then be broken to release the cDNAs, which may be combined together for further sequencing, analysis, etc.",

author = "Weitz, \{David A.\} and Huidan Zhang and CUI, \{Nai Wen\} and Yamei Cai and Peter Girguis and Frank Stewart and Neha Sarode and Beate Kraft",

year = "2018",

month = apr,

day = "12",

language = "English",

type = "Patent",

note = "WO 2018/067792 A1; C12N 15/10 (2006.01),C12Q 1/68 (2006 .01)",

}

TY - PAT

T1 - Sequencing of bacteria or other species

AU - Weitz, David A.

AU - Zhang, Huidan

AU - CUI, Nai Wen

AU - Cai, Yamei

AU - Girguis, Peter

AU - Stewart, Frank

AU - Sarode, Neha

AU - Kraft, Beate

PY - 2018/4/12

Y1 - 2018/4/12

N2 - The present invention generally relates to sequencing of cells such as bacteria or archeons. In one aspect, cells are lysed to release RNA, which is then polyadenylated and exposed to a sequencing having an identification portion and a poly-T portion, which associates with the RNA. These are then reverse transcribed to produce cDNA, which can then be studied, amplified, etc. In some cases, such reactions may be performed within droplets. The droplets can then be broken to release the cDNAs, which may be combined together for further sequencing, analysis, etc.

AB - The present invention generally relates to sequencing of cells such as bacteria or archeons. In one aspect, cells are lysed to release RNA, which is then polyadenylated and exposed to a sequencing having an identification portion and a poly-T portion, which associates with the RNA. These are then reverse transcribed to produce cDNA, which can then be studied, amplified, etc. In some cases, such reactions may be performed within droplets. The droplets can then be broken to release the cDNAs, which may be combined together for further sequencing, analysis, etc.

M3 - Patent

M1 - WO 2018/067792 A1

Y2 - 2017/10/05

ER -

Sequencing of bacteria or other species

Abstract

SDU Link

Fingerprint

Cite this