Sequencing of bacteria or other species

David A. Weitz (Inventor), Huidan Zhang (Inventor), Nai Wen CUI (Inventor), Yamei Cai (Inventor), Peter Girguis (Inventor), Frank Stewart (Inventor), Neha Sarode (Inventor), Beate Kraft (Inventor)

Research output: Patent


The present invention generally relates to sequencing of cells such as bacteria or archeons. In one aspect, cells are lysed to release RNA, which is then polyadenylated and exposed to a sequencing having an identification portion and a poly-T portion, which associates with the RNA. These are then reverse transcribed to produce cDNA, which can then be studied, amplified, etc. In some cases, such reactions may be performed within droplets. The droplets can then be broken to release the cDNAs, which may be combined together for further sequencing, analysis, etc.
Original languageEnglish
IPCC12N 15/10 (2006.01),C12Q 1/68 (2006 .01)
Patent numberWO 2018/067792 A1
Filing date05/10/2017
Country/TerritoryUnited States
Priority date01/07/2016
Priority numberUS201662405775P
Publication statusPublished - 12. Apr 2018


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