The present invention generally relates to sequencing of cells such as bacteria or archeons. In one aspect, cells are lysed to release RNA, which is then polyadenylated and exposed to a sequencing having an identification portion and a poly-T portion, which associates with the RNA. These are then reverse transcribed to produce cDNA, which can then be studied, amplified, etc. In some cases, such reactions may be performed within droplets. The droplets can then be broken to release the cDNAs, which may be combined together for further sequencing, analysis, etc.
|IPC||C12N 15/10 (2006.01),C12Q 1/68 (2006 .01)|
|Patent number||WO 2018/067792 A1|
|Publication status||Published - 12. Apr 2018|