Role of B-cells in mycosis fungoides

Pia Rude Nielsen; Jens Ole Eriksen; Mia Dahl Sørensen; Ulrike Wehkamp; Lise Maria Lindahl; Michael Bzorek; Lars Iversen; Anders Woetman; Niels Ødum; Thomas Litman; Lise Mette Rahbek Gjerdrum

doi:10.2340/00015555-3775

Role of B-cells in mycosis fungoides

Pia Rude Nielsen^*
, Jens Ole Eriksen
, Mia Dahl Sørensen
, Ulrike Wehkamp
, Lise Maria Lindahl
, Michael Bzorek
, Lars Iversen
, Anders Woetman
, Niels Ødum
, Thomas Litman
, Lise Mette Rahbek Gjerdrum

^*Corresponding author for this work

KI, OUH, Research unit of Pathology (Odense)

Zealand University Hospital, Roskilde
Aarhus University Hospital
University of Copenhagen
University Medical Center Schleswig-Holstein

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Mycosis fungoides is the most common type of cutaneous T-cell lymphoma. The inflammatory microenvironment in mycosis fungoides is complex. There is accumulating evidence that the neoplastic T-cells take control of the microenvironment and thereby promote their own expansion by suppressing cellular immunity. B-cells have proved to be upregulated in large-cell transformed mycosis fungoides, and could potentially play a role in disease progression. To investigate the presence of B-cells in mycosis fungoides compared with controls, this study analysed 85 formalin-fixed and paraffin-embedded mycosis fungoides biopsies. MS4A1 gene expression was significantly upregulated in mycosis fungoides compared with controls (p < 0.0001) and further upregulated in disease progression, (p = 0.001). Digital quantification of PAX5⁺/ CD20⁺ cells confirmed the increased presence of Bcells in mycosis fungoides compared with controls. No co-labelling of CD3/CD20 was observed in the neoplastic T-cells. This study found a significantly increased presence of B-cells in the tumour-associated microenvironment in mycosis fungoides. These findings could potentially lead to new treatment strategies for mycosis fungoides.

Original language	English
Article number	adv00413
Journal	Acta Dermato-Venereologica
Volume	101
Issue number	3
ISSN	0001-5555
DOIs	https://doi.org/10.2340/00015555-3775
Publication status	Published - 11. Mar 2021

Bibliographical note

Publisher Copyright:
© 2021, Medical Journals/Acta D-V. All rights reserved.

Funding

LMRG has received funding from NanoString Technologies. T.L. is employed both by Copenhagen University and by LEO Pharma A/S. The remaining authors have no conflicts of interest to declare. This work was supported by the Danish Cancer Research Foundation (Dansk Kræftforskningsfond), Region Zealand Health and Research Foundation, the Region of Zealand and Southern Denmark Research Foundation, Aage Bang Foundation, LINAK A/S Nordborg, the Harboe Foundation, the carpenter Jørgen Holm and wife Elisa F. Hansen Memorial Foundation, the Medical Research Association Foundation and the A.P. Moeller Foundation. The technical assistance provided by the immunohistochemical and molecular team at the Department of Pathology, N?stved, Denmark is greatly appreciated. This work was supported by the Danish Cancer Research Foundation (Dansk Kr?ftforskningsfond), Region Zealand Health and Research Foundation, the Region of Zealand and Southern Denmark Research Foundation, Aage Bang Foundation, LINAK A/S Nordborg, the Harboe Foundation, the carpenter J?rgen Holm and wife Elisa F. Hansen Memorial Foundation, the Medical Research Association Foundation and the A.P. Moeller Foundation. LMRG has received funding from NanoString Technologies. T.L. is employed both by Copenhagen University and by LEO Pharma A/S. The remaining authors have no conflicts of interest to declare.

Keywords

B-cells
Cutaneous t-cell lymphoma
Mycosis fungoides
Tumour microenvironment

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Open Access VersionFinal published version, 3.25 MBLicence: CC BY-NC

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title = "Role of B-cells in mycosis fungoides",

abstract = "Mycosis fungoides is the most common type of cutaneous T-cell lymphoma. The inflammatory microenvironment in mycosis fungoides is complex. There is accumulating evidence that the neoplastic T-cells take control of the microenvironment and thereby promote their own expansion by suppressing cellular immunity. B-cells have proved to be upregulated in large-cell transformed mycosis fungoides, and could potentially play a role in disease progression. To investigate the presence of B-cells in mycosis fungoides compared with controls, this study analysed 85 formalin-fixed and paraffin-embedded mycosis fungoides biopsies. MS4A1 gene expression was significantly upregulated in mycosis fungoides compared with controls (p < 0.0001) and further upregulated in disease progression, (p = 0.001). Digital quantification of PAX5+/ CD20+ cells confirmed the increased presence of Bcells in mycosis fungoides compared with controls. No co-labelling of CD3/CD20 was observed in the neoplastic T-cells. This study found a significantly increased presence of B-cells in the tumour-associated microenvironment in mycosis fungoides. These findings could potentially lead to new treatment strategies for mycosis fungoides.",

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AU - Nielsen, Pia Rude

AU - Eriksen, Jens Ole

AU - Sørensen, Mia Dahl

AU - Wehkamp, Ulrike

AU - Lindahl, Lise Maria

AU - Bzorek, Michael

AU - Iversen, Lars

AU - Woetman, Anders

AU - Ødum, Niels

AU - Litman, Thomas

AU - Gjerdrum, Lise Mette Rahbek

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N2 - Mycosis fungoides is the most common type of cutaneous T-cell lymphoma. The inflammatory microenvironment in mycosis fungoides is complex. There is accumulating evidence that the neoplastic T-cells take control of the microenvironment and thereby promote their own expansion by suppressing cellular immunity. B-cells have proved to be upregulated in large-cell transformed mycosis fungoides, and could potentially play a role in disease progression. To investigate the presence of B-cells in mycosis fungoides compared with controls, this study analysed 85 formalin-fixed and paraffin-embedded mycosis fungoides biopsies. MS4A1 gene expression was significantly upregulated in mycosis fungoides compared with controls (p < 0.0001) and further upregulated in disease progression, (p = 0.001). Digital quantification of PAX5+/ CD20+ cells confirmed the increased presence of Bcells in mycosis fungoides compared with controls. No co-labelling of CD3/CD20 was observed in the neoplastic T-cells. This study found a significantly increased presence of B-cells in the tumour-associated microenvironment in mycosis fungoides. These findings could potentially lead to new treatment strategies for mycosis fungoides.

AB - Mycosis fungoides is the most common type of cutaneous T-cell lymphoma. The inflammatory microenvironment in mycosis fungoides is complex. There is accumulating evidence that the neoplastic T-cells take control of the microenvironment and thereby promote their own expansion by suppressing cellular immunity. B-cells have proved to be upregulated in large-cell transformed mycosis fungoides, and could potentially play a role in disease progression. To investigate the presence of B-cells in mycosis fungoides compared with controls, this study analysed 85 formalin-fixed and paraffin-embedded mycosis fungoides biopsies. MS4A1 gene expression was significantly upregulated in mycosis fungoides compared with controls (p < 0.0001) and further upregulated in disease progression, (p = 0.001). Digital quantification of PAX5+/ CD20+ cells confirmed the increased presence of Bcells in mycosis fungoides compared with controls. No co-labelling of CD3/CD20 was observed in the neoplastic T-cells. This study found a significantly increased presence of B-cells in the tumour-associated microenvironment in mycosis fungoides. These findings could potentially lead to new treatment strategies for mycosis fungoides.

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KW - Cutaneous t-cell lymphoma

KW - Mycosis fungoides

KW - Tumour microenvironment

U2 - 10.2340/00015555-3775

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M3 - Journal article

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SN - 0001-5555

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ER -

Role of B-cells in mycosis fungoides

Abstract

Bibliographical note

Funding

Keywords

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