Reverse Phase Protein Arrays for High-Throughput Protein Measurements in Mammospheres

Marlene Lemvig Pedersen, Ines Block, Markus List, Leena Karimi, Helle Christiansen, Steffen Schmidt, Jan Mollenhauer

    Research output: Contribution to conference without publisher/journalPosterResearch

    Abstract

    Short interfering RNAs (siRNAs) are an attractive option for the next generation of anti-cancer drugs, due to their ability to selectively inactivate each of the 20,000 human genes. Suitable siRNAs for the development of such drugs are commonly recovered via automated genome-wide siRNA screens. In a differential screen, comparing e.g. cancer cells versus normal cells, up to 120,000 cell experiments are performed in a highly parallelized fashion. The readout options of such high-throughput screens are, however, generally limited to one or very few parameters.
    We report on establishing a Reverse Phase Protein Array (RPPA)-based readout format integrated into robotic siRNA screening. This technique would allow post-screening high-throughput quantification of protein changes.
    Recently, breast cancer stem cells (BCSCs) have attracted much attention, as a tumor- and metastasis-driving subpopulation, and thus this methodology was laid out for and tested using mammospheres which are believed to be enriched for BCSCs.
    Original languageEnglish
    Publication date10. Mar 2014
    Publication statusPublished - 10. Mar 2014
    EventAnnual Conference for Advances in Microarray Technology - Berlin, Germany
    Duration: 10. Mar 201411. Mar 2014
    Conference number: 8th

    Conference

    ConferenceAnnual Conference for Advances in Microarray Technology
    Number8th
    CountryGermany
    CityBerlin
    Period10/03/201411/03/2014

    Fingerprint

    Protein Array Analysis
    Small Interfering RNA
    Proteins
    Neoplasms
    Robotics
    Pharmaceutical Preparations

    Cite this

    Pedersen, M. L., Block, I., List, M., Karimi, L., Christiansen, H., Schmidt, S., & Mollenhauer, J. (2014). Reverse Phase Protein Arrays for High-Throughput Protein Measurements in Mammospheres. Poster session presented at Annual Conference for Advances in Microarray Technology, Berlin, Germany.
    Pedersen, Marlene Lemvig ; Block, Ines ; List, Markus ; Karimi, Leena ; Christiansen, Helle ; Schmidt, Steffen ; Mollenhauer, Jan. / Reverse Phase Protein Arrays for High-Throughput Protein Measurements in Mammospheres. Poster session presented at Annual Conference for Advances in Microarray Technology, Berlin, Germany.
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    title = "Reverse Phase Protein Arrays for High-Throughput Protein Measurements in Mammospheres",
    abstract = "Short interfering RNAs (siRNAs) are an attractive option for the next generation of anti-cancer drugs, due to their ability to selectively inactivate each of the 20,000 human genes. Suitable siRNAs for the development of such drugs are commonly recovered via automated genome-wide siRNA screens. In a differential screen, comparing e.g. cancer cells versus normal cells, up to 120,000 cell experiments are performed in a highly parallelized fashion. The readout options of such high-throughput screens are, however, generally limited to one or very few parameters.We report on establishing a Reverse Phase Protein Array (RPPA)-based readout format integrated into robotic siRNA screening. This technique would allow post-screening high-throughput quantification of protein changes. Recently, breast cancer stem cells (BCSCs) have attracted much attention, as a tumor- and metastasis-driving subpopulation, and thus this methodology was laid out for and tested using mammospheres which are believed to be enriched for BCSCs.",
    author = "Pedersen, {Marlene Lemvig} and Ines Block and Markus List and Leena Karimi and Helle Christiansen and Steffen Schmidt and Jan Mollenhauer",
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    Pedersen, ML, Block, I, List, M, Karimi, L, Christiansen, H, Schmidt, S & Mollenhauer, J 2014, 'Reverse Phase Protein Arrays for High-Throughput Protein Measurements in Mammospheres' Annual Conference for Advances in Microarray Technology, Berlin, Germany, 10/03/2014 - 11/03/2014, .

    Reverse Phase Protein Arrays for High-Throughput Protein Measurements in Mammospheres. / Pedersen, Marlene Lemvig; Block, Ines; List, Markus; Karimi, Leena; Christiansen, Helle; Schmidt, Steffen; Mollenhauer, Jan.

    2014. Poster session presented at Annual Conference for Advances in Microarray Technology, Berlin, Germany.

    Research output: Contribution to conference without publisher/journalPosterResearch

    TY - CONF

    T1 - Reverse Phase Protein Arrays for High-Throughput Protein Measurements in Mammospheres

    AU - Pedersen, Marlene Lemvig

    AU - Block, Ines

    AU - List, Markus

    AU - Karimi, Leena

    AU - Christiansen, Helle

    AU - Schmidt, Steffen

    AU - Mollenhauer, Jan

    PY - 2014/3/10

    Y1 - 2014/3/10

    N2 - Short interfering RNAs (siRNAs) are an attractive option for the next generation of anti-cancer drugs, due to their ability to selectively inactivate each of the 20,000 human genes. Suitable siRNAs for the development of such drugs are commonly recovered via automated genome-wide siRNA screens. In a differential screen, comparing e.g. cancer cells versus normal cells, up to 120,000 cell experiments are performed in a highly parallelized fashion. The readout options of such high-throughput screens are, however, generally limited to one or very few parameters.We report on establishing a Reverse Phase Protein Array (RPPA)-based readout format integrated into robotic siRNA screening. This technique would allow post-screening high-throughput quantification of protein changes. Recently, breast cancer stem cells (BCSCs) have attracted much attention, as a tumor- and metastasis-driving subpopulation, and thus this methodology was laid out for and tested using mammospheres which are believed to be enriched for BCSCs.

    AB - Short interfering RNAs (siRNAs) are an attractive option for the next generation of anti-cancer drugs, due to their ability to selectively inactivate each of the 20,000 human genes. Suitable siRNAs for the development of such drugs are commonly recovered via automated genome-wide siRNA screens. In a differential screen, comparing e.g. cancer cells versus normal cells, up to 120,000 cell experiments are performed in a highly parallelized fashion. The readout options of such high-throughput screens are, however, generally limited to one or very few parameters.We report on establishing a Reverse Phase Protein Array (RPPA)-based readout format integrated into robotic siRNA screening. This technique would allow post-screening high-throughput quantification of protein changes. Recently, breast cancer stem cells (BCSCs) have attracted much attention, as a tumor- and metastasis-driving subpopulation, and thus this methodology was laid out for and tested using mammospheres which are believed to be enriched for BCSCs.

    M3 - Poster

    ER -

    Pedersen ML, Block I, List M, Karimi L, Christiansen H, Schmidt S et al. Reverse Phase Protein Arrays for High-Throughput Protein Measurements in Mammospheres. 2014. Poster session presented at Annual Conference for Advances in Microarray Technology, Berlin, Germany.