Responsive Emulsions for Sequential Multienzyme Cascades

Zhiyong Sun, Qingcai Zhao, Rainer Haag*, Changzhu Wu*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the reaction equilibrium to the product side. However, multienzyme systems are often limited by their incompatibility and cross-reactivity. Herein, we report a multi-responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO2, pH, and thermo-responsive block copolymer as a stabilizer, allowing the on-demand control of emulsion morphology and phase composition. Applying this system to a three-step cascade reaction enables the individual optimal condition for each enzyme, and a high overall conversion (ca. 97 % of the calculated limit) is thereby obtained. Moreover, the multi-responsiveness of the emulsion allows the facile and separate yielding/recycling of products, polymers and active enzymes. Besides, the system could be scaled up with a good yield.

Original languageEnglish
JournalAngewandte Chemie - International Edition
Volume60
Issue number15
Pages (from-to)8410-8414
ISSN1433-7851
DOIs
Publication statusPublished - 6. Apr 2021

Keywords

  • biocatalysis
  • block copolymers
  • multienzyme reaction
  • responsive emulsion

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