Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins

Sofie Traynor, Niels Erik Møllegaard, Mikkel G Jørgensen, Nadine H Brückmann, Christina B Pedersen, Mikkel G Terp, Simone Johansen, Jerome Dejardin, Henrik J Ditzel, Morten F Gjerstorff

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Abstract

Rearrangement of the 1q12 pericentromeric heterochromatin and subsequent amplification of the 1q arm is commonly associated with cancer development and progression and may result from epigenetic deregulation. In many premalignant and malignant cells, loss of 1q12 satellite DNA methylation causes the deposition of polycomb factors and formation of large polycomb aggregates referred to as polycomb bodies. Here, we show that SSX proteins can destabilize 1q12 pericentromeric heterochromatin in melanoma cells when it is present in the context of polycomb bodies. We found that SSX proteins deplete polycomb bodies and promote the unfolding and derepression of 1q12 heterochromatin during replication. This further leads to segregation abnormalities during anaphase and generation of micronuclei. The structural rearrangement of 1q12 pericentromeric heterochromatin triggered by SSX2 is associated with loss of polycomb factors, but is not mediated by diminished polycomb repression. Instead, our studies suggest a direct effect of SSX proteins facilitated though a DNA/chromatin binding, zinc finger-like domain and a KRAB-like domain that may recruit chromatin modifiers or activate satellite transcription. Our results demonstrate a novel mechanism for generation of 1q12-associated genomic instability in cancer cells.

Original languageEnglish
JournalNucleic acids research
Volume47
Issue number13
Pages (from-to)6668-6684
ISSN0305-1048
DOIs
Publication statusPublished - 26. Jul 2019

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Chromosomes, Human, Pair 1
Proteins
Satellite DNA
DNA Methylation
Epigenomics
Melanoma
Neoplasms
DNA

Cite this

@article{90a6cb1435d4483ba1831bb230236d33,
title = "Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins",
abstract = "Rearrangement of the 1q12 pericentromeric heterochromatin and subsequent amplification of the 1q arm is commonly associated with cancer development and progression and may result from epigenetic deregulation. In many premalignant and malignant cells, loss of 1q12 satellite DNA methylation causes the deposition of polycomb factors and formation of large polycomb aggregates referred to as polycomb bodies. Here, we show that SSX proteins can destabilize 1q12 pericentromeric heterochromatin in melanoma cells when it is present in the context of polycomb bodies. We found that SSX proteins deplete polycomb bodies and promote the unfolding and derepression of 1q12 heterochromatin during replication. This further leads to segregation abnormalities during anaphase and generation of micronuclei. The structural rearrangement of 1q12 pericentromeric heterochromatin triggered by SSX2 is associated with loss of polycomb factors, but is not mediated by diminished polycomb repression. Instead, our studies suggest a direct effect of SSX proteins facilitated though a DNA/chromatin binding, zinc finger-like domain and a KRAB-like domain that may recruit chromatin modifiers or activate satellite transcription. Our results demonstrate a novel mechanism for generation of 1q12-associated genomic instability in cancer cells.",
author = "Sofie Traynor and M{\o}llegaard, {Niels Erik} and J{\o}rgensen, {Mikkel G} and Br{\"u}ckmann, {Nadine H} and Pedersen, {Christina B} and Terp, {Mikkel G} and Simone Johansen and Jerome Dejardin and Ditzel, {Henrik J} and Gjerstorff, {Morten F}",
note = "{\circledC} The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2019",
month = "7",
day = "26",
doi = "10.1093/nar/gkz396",
language = "English",
volume = "47",
pages = "6668--6684",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Heinemann",
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}

Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins. / Traynor, Sofie; Møllegaard, Niels Erik; Jørgensen, Mikkel G; Brückmann, Nadine H; Pedersen, Christina B; Terp, Mikkel G; Johansen, Simone; Dejardin, Jerome; Ditzel, Henrik J; Gjerstorff, Morten F.

In: Nucleic acids research, Vol. 47, No. 13, 26.07.2019, p. 6668-6684.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins

AU - Traynor, Sofie

AU - Møllegaard, Niels Erik

AU - Jørgensen, Mikkel G

AU - Brückmann, Nadine H

AU - Pedersen, Christina B

AU - Terp, Mikkel G

AU - Johansen, Simone

AU - Dejardin, Jerome

AU - Ditzel, Henrik J

AU - Gjerstorff, Morten F

N1 - © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2019/7/26

Y1 - 2019/7/26

N2 - Rearrangement of the 1q12 pericentromeric heterochromatin and subsequent amplification of the 1q arm is commonly associated with cancer development and progression and may result from epigenetic deregulation. In many premalignant and malignant cells, loss of 1q12 satellite DNA methylation causes the deposition of polycomb factors and formation of large polycomb aggregates referred to as polycomb bodies. Here, we show that SSX proteins can destabilize 1q12 pericentromeric heterochromatin in melanoma cells when it is present in the context of polycomb bodies. We found that SSX proteins deplete polycomb bodies and promote the unfolding and derepression of 1q12 heterochromatin during replication. This further leads to segregation abnormalities during anaphase and generation of micronuclei. The structural rearrangement of 1q12 pericentromeric heterochromatin triggered by SSX2 is associated with loss of polycomb factors, but is not mediated by diminished polycomb repression. Instead, our studies suggest a direct effect of SSX proteins facilitated though a DNA/chromatin binding, zinc finger-like domain and a KRAB-like domain that may recruit chromatin modifiers or activate satellite transcription. Our results demonstrate a novel mechanism for generation of 1q12-associated genomic instability in cancer cells.

AB - Rearrangement of the 1q12 pericentromeric heterochromatin and subsequent amplification of the 1q arm is commonly associated with cancer development and progression and may result from epigenetic deregulation. In many premalignant and malignant cells, loss of 1q12 satellite DNA methylation causes the deposition of polycomb factors and formation of large polycomb aggregates referred to as polycomb bodies. Here, we show that SSX proteins can destabilize 1q12 pericentromeric heterochromatin in melanoma cells when it is present in the context of polycomb bodies. We found that SSX proteins deplete polycomb bodies and promote the unfolding and derepression of 1q12 heterochromatin during replication. This further leads to segregation abnormalities during anaphase and generation of micronuclei. The structural rearrangement of 1q12 pericentromeric heterochromatin triggered by SSX2 is associated with loss of polycomb factors, but is not mediated by diminished polycomb repression. Instead, our studies suggest a direct effect of SSX proteins facilitated though a DNA/chromatin binding, zinc finger-like domain and a KRAB-like domain that may recruit chromatin modifiers or activate satellite transcription. Our results demonstrate a novel mechanism for generation of 1q12-associated genomic instability in cancer cells.

U2 - 10.1093/nar/gkz396

DO - 10.1093/nar/gkz396

M3 - Journal article

C2 - 31114908

VL - 47

SP - 6668

EP - 6684

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 13

ER -