Recent advances in practical methods for liver cell biology: A short overview

Sandra Torres, Zeinab Abdullah, Maximilian J. Brol, Claus Hellerbrand, Mercedes Fernandez, Romina Fiorotto, Sabine Klein, Philipp Königshofer, Christian Liedtke, Sophie Lotersztajn, Yulia A. Nevzorova, Robert Schierwagen, Thomas Reiberger, Frank Erhard Uschner, Frank Tacke, Ralf Weiskirchen, Jonel Trebicka*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.

Original languageEnglish
Article number2027
JournalInternational Journal of Molecular Sciences
Volume21
Issue number6
ISSN1661-6596
DOIs
Publication statusPublished - 2020

Keywords

  • Fibrogenesis
  • Hepatic stellate cells
  • Hepatocellular cancer
  • In vitro models
  • Steatosis

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