Reactive microgliosis engages distinct responses by microglial subpopulations after minor central nervous system injury.

Martin Wirenfeldt, Alicia Anne Babcock, Rune Ladeby, Kate Lykke Lambertsen, Frederik Dagnaes-Hansen, Robert Graham Quinton Leslie, Trevor Owens, Bente Finsen

Research output: Contribution to journalJournal articleResearchpeer-review


Microglia are bone marrow-derived cells that constitute a facultative macrophage population when activated by trauma or pathology in the CNS. Endogenous CNS-resident microglia as well as exogenous (immigrant) bone marrow-derived cells contribute to reactive microgliosis, raising fundamental questions about the cellular composition, kinetics, and functional characteristics of the reactive microglial cell population. Bone marrow chimeric mice reconstituted with green fluorescent protein-expressing (GFP(+)) donor bone marrow cells were subjected to entorhinal cortex lesion, resulting in selective axonal degeneration and a localized microglial reaction in the hippocampus. Flow cytometric evaluation of individually dissected hippocampi differentiated immigrant GFP(+) microglia from resident GFP(-) microglia (CD11b(+)CD45(dim)) and identified a subset of mainly resident CD11b(+) microglia that was induced to express CD34. The proportion of immigrant GFP(+) microglia (CD11b(+)CD45(dim)) increased signficantly by 3 and 5 days postlesion and reached a maximum of 13% by 7 days. These cells expressed lower CD11b levels than resident microglia, forming a distinct subpopulation on CD11b/CD45 profiles. The proportion of CD34(+)CD11b(+) microglia was significantly increased at 3 days postlesion but had normalized by 5 and 7 days, when the microglial reaction is known to be at its maximum. Our results show that distinct subpopulations of microglia respond to minor CNS injury. The heterogeneity in microglial response may have functional consequences for repair and possibly therapy.
Original languageEnglish
JournalJournal of Neuroscience Research
Issue number4
Pages (from-to)507-14
Number of pages7
Publication statusPublished - 15. Nov 2005


  • Animals
  • Antigens, CD11b
  • Antigens, CD34
  • Bone Marrow Transplantation
  • Cell Movement
  • Central Nervous System Diseases
  • Flow Cytometry
  • Green Fluorescent Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microglia
  • Time Factors


Dive into the research topics of 'Reactive microgliosis engages distinct responses by microglial subpopulations after minor central nervous system injury.'. Together they form a unique fingerprint.

Cite this