Protocol for isolation of human bone marrow stromal cells and characterization of cellular metabolism

Martina Dzubanova; Michaela Ferencakova; Andrea Benova; Dalia Ali; Michaela Tencerova

doi:10.1016/j.xpro.2024.103553

Protocol for isolation of human bone marrow stromal cells and characterization of cellular metabolism

Martina Dzubanova
, Michaela Ferencakova
, Andrea Benova
, Dalia Ali
, Michaela Tencerova^*

^*Corresponding author for this work

The Czech Academy of Sciences
Charles University

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Bone marrow stromal cells (BMSCs) serve as a valuable reservoir of multipotent stem cells important in the regulation of bone homeostasis and energy metabolism. Here, we present a protocol for isolating human BMSCs (hBMSCs) and characterizing their cellular metabolism related to hBMSC functional properties. We describe steps for bioenergetics, cell senescence, and production of reactive oxygen species (ROS), together with description of the data analysis. These assays provide information on hBMSC metabolic status valuable to regenerative medicine and therapeutic applications. For complete details on the use and execution of this protocol, please refer to Tencerova et al.¹

Original language	English
Article number	103553
Journal	STAR Protocols
Volume	6
Issue number	1
Number of pages	26
ISSN	2666-1667
DOIs	https://doi.org/10.1016/j.xpro.2024.103553
Publication status	Published - 21. Mar 2025

Keywords

Cell Biology
Metabolism
Stem Cells

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10.1016/j.xpro.2024.103553Licence: CC BY

Open Access VersionFinal published version, 7.2 MBLicence: CC BY

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title = "Protocol for isolation of human bone marrow stromal cells and characterization of cellular metabolism",

abstract = "Bone marrow stromal cells (BMSCs) serve as a valuable reservoir of multipotent stem cells important in the regulation of bone homeostasis and energy metabolism. Here, we present a protocol for isolating human BMSCs (hBMSCs) and characterizing their cellular metabolism related to hBMSC functional properties. We describe steps for bioenergetics, cell senescence, and production of reactive oxygen species (ROS), together with description of the data analysis. These assays provide information on hBMSC metabolic status valuable to regenerative medicine and therapeutic applications. For complete details on the use and execution of this protocol, please refer to Tencerova et al.1",

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TY - JOUR

T1 - Protocol for isolation of human bone marrow stromal cells and characterization of cellular metabolism

AU - Dzubanova, Martina

AU - Ferencakova, Michaela

AU - Benova, Andrea

AU - Ali, Dalia

AU - Tencerova, Michaela

PY - 2025/3/21

Y1 - 2025/3/21

N2 - Bone marrow stromal cells (BMSCs) serve as a valuable reservoir of multipotent stem cells important in the regulation of bone homeostasis and energy metabolism. Here, we present a protocol for isolating human BMSCs (hBMSCs) and characterizing their cellular metabolism related to hBMSC functional properties. We describe steps for bioenergetics, cell senescence, and production of reactive oxygen species (ROS), together with description of the data analysis. These assays provide information on hBMSC metabolic status valuable to regenerative medicine and therapeutic applications. For complete details on the use and execution of this protocol, please refer to Tencerova et al.1

AB - Bone marrow stromal cells (BMSCs) serve as a valuable reservoir of multipotent stem cells important in the regulation of bone homeostasis and energy metabolism. Here, we present a protocol for isolating human BMSCs (hBMSCs) and characterizing their cellular metabolism related to hBMSC functional properties. We describe steps for bioenergetics, cell senescence, and production of reactive oxygen species (ROS), together with description of the data analysis. These assays provide information on hBMSC metabolic status valuable to regenerative medicine and therapeutic applications. For complete details on the use and execution of this protocol, please refer to Tencerova et al.1

KW - Cell Biology

KW - Metabolism

KW - Stem Cells

U2 - 10.1016/j.xpro.2024.103553

DO - 10.1016/j.xpro.2024.103553

M3 - Journal article

C2 - 39813119

AN - SCOPUS:85214788410

SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 103553

ER -

Protocol for isolation of human bone marrow stromal cells and characterization of cellular metabolism

Abstract

Keywords

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