Proteomics of Fuchs' Endothelial Corneal Dystrophy support that the extracellular matrix of Descemet's membrane is disordered

Ebbe Toftgaard Poulsen, Thomas F Dyrlund, Kasper Runager, Carsten Scavenius, Toke Peter Krogager, Peter Højrup, Ida B Thøgersen, Kristian Wejse Sanggaard, Henrik Vorum, Jesper Hjortdal, Jan Johannes Enghild

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Abstract

Fuchs' endothelial corneal dystrophy (FECD) is a major corneal disorder affecting the innermost part of the cornea, leading to visual impairment. As the morphological changes in FECD are mainly observed in the extracellular matrix of the Descemet's membrane/endothelial layer we determined the protein profiles of diseased and control tissues using two relative quantitation MS methods. The first quantitation method based on the areas of the extracted ion chromatograms, quantified the 51 and 48 most abundant proteins of the Descemet's membrane/endothelial layer in patient and control tissues, respectively, of which 10 were significantly regulated. The results indicated that the level of type VIII collagen was unaltered even though the protein previously has been implicated in familial early onset forms of the disease. Using the second relative quantitation method iTRAQ we identified 22 differentially regulated proteins, many of which are extracellular proteins known to be involved in proper assembly of the basement membrane in other tissues. In total 26 differentially regulated proteins were identified, of which 6 proteins were regulated by both methods. These results support that the morphological changes observed in FECD is caused in part by an aberrant assembly of the extracellular matrix within the Descemet's membrane/endothelial layer.

Original languageEnglish
JournalJournal of Proteome Research
Volume13
Issue number11
Pages (from-to)4659–4667
ISSN1535-3893
DOIs
Publication statusPublished - 2014

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Fuchs' Endothelial Dystrophy
Descemet Membrane
Membranes
Proteins
Tissue
Collagen Type VIII
Proteomics
Membrane Proteins
Ions

Bibliographical note

Publication Date (Web): May 21, 2014

Cite this

Poulsen, Ebbe Toftgaard ; Dyrlund, Thomas F ; Runager, Kasper ; Scavenius, Carsten ; Krogager, Toke Peter ; Højrup, Peter ; Thøgersen, Ida B ; Sanggaard, Kristian Wejse ; Vorum, Henrik ; Hjortdal, Jesper ; Enghild, Jan Johannes. / Proteomics of Fuchs' Endothelial Corneal Dystrophy support that the extracellular matrix of Descemet's membrane is disordered. In: Journal of Proteome Research. 2014 ; Vol. 13, No. 11. pp. 4659–4667.
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title = "Proteomics of Fuchs' Endothelial Corneal Dystrophy support that the extracellular matrix of Descemet's membrane is disordered",
abstract = "Fuchs' endothelial corneal dystrophy (FECD) is a major corneal disorder affecting the innermost part of the cornea, leading to visual impairment. As the morphological changes in FECD are mainly observed in the extracellular matrix of the Descemet's membrane/endothelial layer we determined the protein profiles of diseased and control tissues using two relative quantitation MS methods. The first quantitation method based on the areas of the extracted ion chromatograms, quantified the 51 and 48 most abundant proteins of the Descemet's membrane/endothelial layer in patient and control tissues, respectively, of which 10 were significantly regulated. The results indicated that the level of type VIII collagen was unaltered even though the protein previously has been implicated in familial early onset forms of the disease. Using the second relative quantitation method iTRAQ we identified 22 differentially regulated proteins, many of which are extracellular proteins known to be involved in proper assembly of the basement membrane in other tissues. In total 26 differentially regulated proteins were identified, of which 6 proteins were regulated by both methods. These results support that the morphological changes observed in FECD is caused in part by an aberrant assembly of the extracellular matrix within the Descemet's membrane/endothelial layer.",
author = "Poulsen, {Ebbe Toftgaard} and Dyrlund, {Thomas F} and Kasper Runager and Carsten Scavenius and Krogager, {Toke Peter} and Peter H{\o}jrup and Th{\o}gersen, {Ida B} and Sanggaard, {Kristian Wejse} and Henrik Vorum and Jesper Hjortdal and Enghild, {Jan Johannes}",
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Poulsen, ET, Dyrlund, TF, Runager, K, Scavenius, C, Krogager, TP, Højrup, P, Thøgersen, IB, Sanggaard, KW, Vorum, H, Hjortdal, J & Enghild, JJ 2014, 'Proteomics of Fuchs' Endothelial Corneal Dystrophy support that the extracellular matrix of Descemet's membrane is disordered', Journal of Proteome Research, vol. 13, no. 11, pp. 4659–4667. https://doi.org/10.1021/pr500252r

Proteomics of Fuchs' Endothelial Corneal Dystrophy support that the extracellular matrix of Descemet's membrane is disordered. / Poulsen, Ebbe Toftgaard; Dyrlund, Thomas F; Runager, Kasper; Scavenius, Carsten; Krogager, Toke Peter; Højrup, Peter; Thøgersen, Ida B; Sanggaard, Kristian Wejse; Vorum, Henrik; Hjortdal, Jesper; Enghild, Jan Johannes.

In: Journal of Proteome Research, Vol. 13, No. 11, 2014, p. 4659–4667.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Proteomics of Fuchs' Endothelial Corneal Dystrophy support that the extracellular matrix of Descemet's membrane is disordered

AU - Poulsen, Ebbe Toftgaard

AU - Dyrlund, Thomas F

AU - Runager, Kasper

AU - Scavenius, Carsten

AU - Krogager, Toke Peter

AU - Højrup, Peter

AU - Thøgersen, Ida B

AU - Sanggaard, Kristian Wejse

AU - Vorum, Henrik

AU - Hjortdal, Jesper

AU - Enghild, Jan Johannes

N1 - Publication Date (Web): May 21, 2014

PY - 2014

Y1 - 2014

N2 - Fuchs' endothelial corneal dystrophy (FECD) is a major corneal disorder affecting the innermost part of the cornea, leading to visual impairment. As the morphological changes in FECD are mainly observed in the extracellular matrix of the Descemet's membrane/endothelial layer we determined the protein profiles of diseased and control tissues using two relative quantitation MS methods. The first quantitation method based on the areas of the extracted ion chromatograms, quantified the 51 and 48 most abundant proteins of the Descemet's membrane/endothelial layer in patient and control tissues, respectively, of which 10 were significantly regulated. The results indicated that the level of type VIII collagen was unaltered even though the protein previously has been implicated in familial early onset forms of the disease. Using the second relative quantitation method iTRAQ we identified 22 differentially regulated proteins, many of which are extracellular proteins known to be involved in proper assembly of the basement membrane in other tissues. In total 26 differentially regulated proteins were identified, of which 6 proteins were regulated by both methods. These results support that the morphological changes observed in FECD is caused in part by an aberrant assembly of the extracellular matrix within the Descemet's membrane/endothelial layer.

AB - Fuchs' endothelial corneal dystrophy (FECD) is a major corneal disorder affecting the innermost part of the cornea, leading to visual impairment. As the morphological changes in FECD are mainly observed in the extracellular matrix of the Descemet's membrane/endothelial layer we determined the protein profiles of diseased and control tissues using two relative quantitation MS methods. The first quantitation method based on the areas of the extracted ion chromatograms, quantified the 51 and 48 most abundant proteins of the Descemet's membrane/endothelial layer in patient and control tissues, respectively, of which 10 were significantly regulated. The results indicated that the level of type VIII collagen was unaltered even though the protein previously has been implicated in familial early onset forms of the disease. Using the second relative quantitation method iTRAQ we identified 22 differentially regulated proteins, many of which are extracellular proteins known to be involved in proper assembly of the basement membrane in other tissues. In total 26 differentially regulated proteins were identified, of which 6 proteins were regulated by both methods. These results support that the morphological changes observed in FECD is caused in part by an aberrant assembly of the extracellular matrix within the Descemet's membrane/endothelial layer.

U2 - 10.1021/pr500252r

DO - 10.1021/pr500252r

M3 - Journal article

C2 - 24846694

VL - 13

SP - 4659

EP - 4667

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 11

ER -