Proteome analysis of interleukin-1beta-induced changes in protein expression in rat islets of Langerhans

P M Larsen, S J Fey, M R Larsen, A Nawrocki, H U Andersen, H Kähler, C Heilmann, M C Voss, P Roepstorff, F Pociot, A E Karlsen, J Nerup

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The intracellular molecular events involved in the beta-cell death process are complex but poorly understood. Cytokines, e.g., interleukin (IL)-1beta, may play a crucial role in inducing this process. Protein synthesis is necessary for the deleterious effect of IL-1, and induction of both protective and deleterious proteins has been described. To characterize the rather complex pattern of islet protein expression in rat islets in response to IL-1, we have attempted to identify proteins of altered expression level after IL-1 exposure by 2D gel electrophoresis and mass spectrometry. Of 105 significantly changed (i.e., up- or downregulated or de novo-induced) protein spots, we obtained positive protein identification for 60 protein spots. The 60 identifications corresponded to 57 different proteins. Of these, 10 proteins were present in two to four spots, suggesting that posttranslatory modifications had occurred. In addition, 11 spots contained more than one protein. The proteins could be classified according to their function into the following groups: 1) energy transduction; 2) glycolytic pathway; 3) protein synthesis, chaperones, and protein folding; and 4) signal transduction, regulation, differentiation, and apoptosis. In conclusion, valuable information about the molecular mechanisms involved in cytokine-mediated beta-cell destruction was obtained by this approach.
Original languageEnglish
JournalDiabetes
Volume50
Issue number5
Pages (from-to)1056-63
Number of pages7
ISSN0046-0192
DOIs
Publication statusPublished - 1. May 2001

Keywords

  • Animals
  • Cells, Cultured
  • Electrophoresis, Gel, Two-Dimensional
  • Energy Metabolism
  • Gene Expression Regulation
  • Interleukin-1
  • Islets of Langerhans
  • Mass Spectrometry
  • Oxidation-Reduction
  • Proteins
  • Proteome
  • Rats

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