Prokaryotic diversity and activity in contrasting productivity regimes in late summer in the Kerguelen region (Southern Ocean)

Alejandra Elisa Hernandez-Magana, Yan Liu, Pavla Debeljak, Olivier Crispi, Barbara Marie, Coco Koedooder, Ingrid Obernosterer*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Natural iron (Fe) fertilization sustains phytoplankton blooms above the Kerguelen plateau (Indian sector of the Southern Ocean) within otherwise low productive off-plateau waters. In early spring and summer, these diatom-dominated blooms are associated with distinct heterotrophic prokaryotic communities, but whether a structuring effect extends to the post-bloom period has thus far not been investigated. To address this question, we carried out a detailed study of the prokaryotic community composition in the region of Kerguelen Island during late Austral summer (18 February to 27 March 2018; MOBYDICK project). Concentrations of chlorophyll a were seasonally low above the plateau (0.27–0.58 μg Chl a L−1) and in a similar range to those at the 3 off-plateau sites investigated (0.14–0.34 μg Chl a L−1), but we observed an accumulation of dissolved organic carbon and the build-up of heterotrophic prokaryotic biomass in Kerguelen plateau waters. Illumina sequencing of the 16S rRNA gene revealed that the total (DNA-based) and potentially active (RNA-based) prokaryotic communities were structured according to on- and off-plateau sites in the wind-mixed surface layer, in both the free-living (<0.8 μm size fraction) and particle-attached (>0.8 μm size fraction) fractions. The Amplicon Sequence Variants (ASV) with significantly higher relative abundances in on-plateau surface waters as compared to off-plateau waters belonged to Halieaceae OM60 group, several Flavobacteriaceae, such as the NS5 marine group, Aurantivirga and Ulvibacter, Rhodobacteraceae Loktanella, Saprospiraceae, and the Cryomorphaceae NS10 marine group. ASVs with higher relative abundances in off-plateau waters belonged to the Flavobacteriaceae Formosa, the Rhodobacteraceae Planktomarina and the SAR11 clade. We discuss the potential abiotic and biotic drivers of community composition in late Austral summer and the ecological roles of abundant prokaryotic taxa in Kerguelen plateau waters.

Original languageEnglish
Article number103561
JournalJournal of Marine Systems
Volume221
Number of pages12
ISSN0924-7963
DOIs
Publication statusPublished - Sep 2021

Bibliographical note

Publisher Copyright:
© 2021 Elsevier B.V.

Keywords

  • 16S rRNA
  • Amplicon sequence variants
  • Natural iron fertilization
  • Prokaryotic community composition
  • Southern Ocean

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