Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation

Angel Manteca, Beatriz Rioseras, Adelina Rogowska-Wrzesinska, Ole N Jensen

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

The extension and biological role of Ser/Thr/Tyr phosphorylation in prokaryotes have been only scarcely studied. In this chapter, we describe the state of the art of microbial phosphoproteomics, focusing on protocols used for studying the phosphoproteome of Streptomyces coelicolor, one of the bacteria encoding the largest number of eukaryote-like Ser/Thr/Tyr kinases.

Original languageEnglish
Title of host publicationMicrobial Proteomics : Methods and Protocols
EditorsDörte Becher
Volume1841
PublisherHumana Press
Publication date2018
Pages249-260
ISBN (Print)978-1-4939-8693-4
ISBN (Electronic)978-1-4939-8695-8
DOIs
Publication statusPublished - 2018
SeriesMethods in Molecular Biology
ISSN1064-3745

Keywords

  • Differentiation
  • LC-MS/MS
  • Phosphoproteomics
  • Sporulation
  • Streptomyces

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