Phosphoproteomic Analysis across the Yeast Life Cycle Reveals Control of Fatty Acyl Chain Length by Phosphorylation of the Fatty Acid Synthase Complex

Fernando Martínez-Montañés, Albert Casanovas, Richard R. Sprenger, Magdalena Topolska, David L. Marshall, Marta Moreno-Torres, Berwyck L.J. Poad, Stephen J. Blanksby, Martin Hermansson, Ole N. Jensen, Christer S. Ejsing*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The ability to remodel lipid metabolism under changing conditions is pivotal for cellular functionality and homeostasis. Here, we characterize the regulatory landscape of phosphorylation-based signaling events across the life cycle of Saccharomyces cerevisiae and determine its impact on the regulation of lipid metabolism. Our data show that 50 lipid metabolic proteins are differentially phosphorylated as cells transit between different physiological states. To identify functional phosphosites, we devised a strategy where multiple phosphosites are simultaneously mutated into phosphomimetic or phosphodeficient alleles and mutants are phenotyped by in-depth lipidomics flux analysis. This uncovers functional phosphosites in the phosphatidate cytidylyltransferase Cds1, the phosphatidylserine synthase Cho1, and Fas2, the α-subunit of the fatty acid synthase (FAS) complex. Furthermore, we show that the fatty acyl chain length produced by FAS is governed by phosphorylation. Overall, our work demonstrates a vital role for phosphoregulation of lipid metabolism and provides a resource to investigate its molecular underpinnings.

Original languageEnglish
Article number108024
JournalCell Reports
Volume32
Issue number6
ISSN2211-1247
DOIs
Publication statusPublished - 11. Aug 2020

Keywords

  • FAS
  • fatty acid synthase
  • fatty acyl chain-length control
  • flux analysis
  • lipid metabolism
  • lipidomics
  • phosphoproteomics
  • Saccharomyces cerevisiae
  • signaling pathways

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