OSCAR is a collagen receptor that costimulates osteoclastogenesis in DAP12-deficient humans and mice

Alexander David Barrow, Nicolas Raynal, Thomas Levin Andersen, David A Slatter, Dominique Bihan, Nicholas Pugh, Marina Cella, Taesoo Kim, Jaerang Rho, Takako Negishi-Koga, Jean-Marie Delaisse, Hiroshi Takayanagi, Joseph Lorenzo, Marco Colonna, Richard W Farndale, Yongwon Choi, John Trowsdale

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Osteoclasts are terminally differentiated leukocytes that erode the mineralized bone matrix. Osteoclastogenesis requires costimulatory receptor signaling through adaptors containing immunoreceptor tyrosine-based activation motifs (ITAMs), such as Fc receptor common γ (FcRγ) and DNAX-activating protein of 12 kDa. Identification of these ITAM-containing receptors and their ligands remains a high research priority, since the stimuli for osteoclastogenesis are only partly defined. Osteoclast-associated receptor (OSCAR) was proposed to be a potent FcRγ-associated costimulatory receptor expressed by preosteoclasts in vitro, but OSCAR lacks a cognate ligand and its role in vivo has been unclear. Using samples from mice and patients deficient in various ITAM signaling pathways, we show here that OSCAR costimulates one of the major FcRγ-associated pathways required for osteoclastogenesis in vivo. Furthermore, we found that OSCAR binds to specific motifs within fibrillar collagens in the ECM that become revealed on nonquiescent bone surfaces in which osteoclasts undergo maturation and terminal differentiation in vivo. OSCAR promoted osteoclastogenesis in vivo, and OSCAR binding to its collagen motif led to signaling that increased numbers of osteoclasts in culture. Thus, our results suggest that ITAM-containing receptors can respond to exposed ligands in collagen, leading to the functional differentiation of leukocytes, which provides what we believe to be a new concept for ITAM regulation of cytokine receptors in different tissue microenvironments.
Original languageEnglish
JournalJournal of Clinical Investigation
Volume121
Issue number9
Pages (from-to)3505-16
Number of pages12
ISSN0021-9738
DOIs
Publication statusPublished - 2011

Fingerprint Dive into the research topics of 'OSCAR is a collagen receptor that costimulates osteoclastogenesis in DAP12-deficient humans and mice'. Together they form a unique fingerprint.

Cite this