TY - JOUR
T1 - Optimization and kinetic analysis of untreated brewers’ spent grain saccharification process via enzymatic hydrolysis
AU - Sibono, Leonardo
AU - Tronci, Stefania
AU - Hajrizaj, Ron
AU - Christensen, Knud Villy
AU - Errico, Massimiliano
AU - Grosso, Massimiliano
PY - 2023/9
Y1 - 2023/9
N2 - The enzymatic hydrolysis of dried brewers’ spent grain without being pretreated was investigated in this work. The enzymatic hydrolysis experiments were carried out using Cellic ® CTec2 as enzymatic complex and the glucose yield was optimized with respect to temperature, solid loading and enzyme loading. The optimization of enzymatic hydrolysis obtained through the response surface methodology was validated experimentally resulting in a glucose yield of 11.71 ± 0.09 g
glucoseg
DM
−1 at 48.6 °C, 6.7 % w/w biomass loading, and 0.22 mL g
DM
−1 as enzyme concentration. The glucose yield obtained corresponds to 44 % of the theoretical one. The temporal profile of glucose concentration was modeled by using the Chrastil's model, and by a modified version of its classical formulation. The models were compared with respect to the fit goodness and precision of parameter estimation. Even if both models were able to properly describe the transient behavior, the proposed modified Chrastil's model provided a more precise parameter estimation compared to the classical one.
AB - The enzymatic hydrolysis of dried brewers’ spent grain without being pretreated was investigated in this work. The enzymatic hydrolysis experiments were carried out using Cellic ® CTec2 as enzymatic complex and the glucose yield was optimized with respect to temperature, solid loading and enzyme loading. The optimization of enzymatic hydrolysis obtained through the response surface methodology was validated experimentally resulting in a glucose yield of 11.71 ± 0.09 g
glucoseg
DM
−1 at 48.6 °C, 6.7 % w/w biomass loading, and 0.22 mL g
DM
−1 as enzyme concentration. The glucose yield obtained corresponds to 44 % of the theoretical one. The temporal profile of glucose concentration was modeled by using the Chrastil's model, and by a modified version of its classical formulation. The models were compared with respect to the fit goodness and precision of parameter estimation. Even if both models were able to properly describe the transient behavior, the proposed modified Chrastil's model provided a more precise parameter estimation compared to the classical one.
KW - Chrastil´s model
KW - Enzymatic hydrolysis
KW - Enzymatic kinetics
KW - Lignocellulosic biomass
KW - Response surface methodology
U2 - 10.1016/j.bej.2023.109044
DO - 10.1016/j.bej.2023.109044
M3 - Journal article
SN - 1369-703X
VL - 198
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
M1 - 109044
ER -