On the mechanism of oscillations in neutrophils

Jens Christian Brasen, Torben Barington, Lars Folke Olsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We have investigated the regulation of the oscillatory generation of H(2)O(2) and oscillations in shape and size in neutrophils in suspension. The oscillations are independent of cell density and hence do not represent a collective phenomena. Furthermore, the oscillations are independent of the external glucose concentration and the oscillations in H(2)O(2) production are 180 degrees out of phase with the oscillations in NAD(P)H. Cytochalasin B blocked the oscillations in shape and size whereas it increased the period of the oscillations in H(2)O(2) production. 1- and 2-butanol also blocked the oscillations in shape and size, but only 1-butanol inhibited the oscillations in H(2)O(2) production. We conjecture that the oscillations are likely to be due to feedback regulations in the signal transduction cascade involving phosphoinositide 3-kinases (PI3K). We have tested this using a simple mathematical model, which explains most of our experimental observations.
Original languageEnglish
JournalBiophysical Chemistry
Volume148
Issue number1-3
Pages (from-to)82-92
Number of pages10
ISSN0301-4622
DOIs
Publication statusPublished - 1. May 2010

Fingerprint Dive into the research topics of 'On the mechanism of oscillations in neutrophils'. Together they form a unique fingerprint.

Cite this