Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

Chenguang Lou, Simone V Samuelsen, Niels Johan Christensen, Birte Vester, Jesper Wengel

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Mono- and diaminated 2'-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.

Original languageEnglish
JournalBioconjugate Chemistry
Volume28
Issue number4
Pages (from-to)1214-1220
ISSN1043-1802
DOIs
Publication statusPublished - 2017

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Complementary RNA
Oligonucleotides
Nucleotides
RNA
DNA
Stabilization
Complementary DNA
Monomers
Molecular modeling
Nucleic acids
Static Electricity
Nucleic Acids
Electrostatics
amino-LNA

Keywords

  • Journal Article

Cite this

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title = "Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA",
abstract = "Mono- and diaminated 2'-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.",
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Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA. / Lou, Chenguang; Samuelsen, Simone V; Christensen, Niels Johan; Vester, Birte; Wengel, Jesper.

In: Bioconjugate Chemistry, Vol. 28, No. 4, 2017, p. 1214-1220.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

AU - Lou, Chenguang

AU - Samuelsen, Simone V

AU - Christensen, Niels Johan

AU - Vester, Birte

AU - Wengel, Jesper

PY - 2017

Y1 - 2017

N2 - Mono- and diaminated 2'-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.

AB - Mono- and diaminated 2'-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.

KW - Journal Article

U2 - 10.1021/acs.bioconjchem.7b00061

DO - 10.1021/acs.bioconjchem.7b00061

M3 - Journal article

C2 - 28332825

VL - 28

SP - 1214

EP - 1220

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 4

ER -