Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

Chenguang Lou; Simone V Samuelsen; Niels Johan Christensen; Birte Vester; Jesper Wengel

doi:10.1021/acs.bioconjchem.7b00061

Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

Chenguang Lou, Simone V Samuelsen, Niels Johan Christensen, Birte Vester, Jesper Wengel

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Mono- and diaminated 2'-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.

Original language	English
Journal	Bioconjugate Chemistry
Volume	28
Issue number	4
Pages (from-to)	1214-1220
ISSN	1043-1802
DOIs	https://doi.org/10.1021/acs.bioconjchem.7b00061
Publication status	Published - 2017

Keywords

Journal Article

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10.1021/acs.bioconjchem.7b00061

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AU - Lou, Chenguang

AU - Samuelsen, Simone V

AU - Christensen, Niels Johan

AU - Vester, Birte

AU - Wengel, Jesper

PY - 2017

Y1 - 2017

N2 - Mono- and diaminated 2'-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.

AB - Mono- and diaminated 2'-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.

KW - Journal Article

U2 - 10.1021/acs.bioconjchem.7b00061

DO - 10.1021/acs.bioconjchem.7b00061

M3 - Journal article

C2 - 28332825

SN - 1043-1802

VL - 28

SP - 1214

EP - 1220

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 4

ER -

Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

Abstract

Keywords

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