Oligonucleotides containing a piperazino-modified 2'-amino-LNA monomer exhibit very high duplex stability and remarkable nuclease resistance

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Abstract

Incorporation of a piperazino-modified 2'-amino-LNA monomer (PipLNA-T) into oligonucleotides conferred very high affinity and base-pairing selectivity towards complementary DNA and RNA strands. Furthermore, one PipLNA-T modification provided a robust nuclease resistance that safeguarded three neighbouring natural nucleosides from 3'-exonucleolytic degradation. These favourable properties render PipLNA-T a promising oligonucleotide modification for various biological applications.

Original languageEnglish
JournalChemical Communications
Volume51
Issue number19
Pages (from-to)4024-4027
ISSN1359-7345
DOIs
Publication statusPublished - 9. Feb 2015

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Oligonucleotides
Monomers
Complementary RNA
RNA
Nucleosides
DNA
Complementary DNA
Degradation
amino-LNA

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title = "Oligonucleotides containing a piperazino-modified 2'-amino-LNA monomer exhibit very high duplex stability and remarkable nuclease resistance",
abstract = "Incorporation of a piperazino-modified 2'-amino-LNA monomer (PipLNA-T) into oligonucleotides conferred very high affinity and base-pairing selectivity towards complementary DNA and RNA strands. Furthermore, one PipLNA-T modification provided a robust nuclease resistance that safeguarded three neighbouring natural nucleosides from 3'-exonucleolytic degradation. These favourable properties render PipLNA-T a promising oligonucleotide modification for various biological applications.",
author = "Chenguang Lou and Birte Vester and Jesper Wengel",
year = "2015",
month = "2",
day = "9",
doi = "10.1039/c5cc00322a",
language = "English",
volume = "51",
pages = "4024--4027",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "19",

}

Oligonucleotides containing a piperazino-modified 2'-amino-LNA monomer exhibit very high duplex stability and remarkable nuclease resistance. / Lou, Chenguang; Vester, Birte; Wengel, Jesper.

In: Chemical Communications, Vol. 51, No. 19, 09.02.2015, p. 4024-4027.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Oligonucleotides containing a piperazino-modified 2'-amino-LNA monomer exhibit very high duplex stability and remarkable nuclease resistance

AU - Lou, Chenguang

AU - Vester, Birte

AU - Wengel, Jesper

PY - 2015/2/9

Y1 - 2015/2/9

N2 - Incorporation of a piperazino-modified 2'-amino-LNA monomer (PipLNA-T) into oligonucleotides conferred very high affinity and base-pairing selectivity towards complementary DNA and RNA strands. Furthermore, one PipLNA-T modification provided a robust nuclease resistance that safeguarded three neighbouring natural nucleosides from 3'-exonucleolytic degradation. These favourable properties render PipLNA-T a promising oligonucleotide modification for various biological applications.

AB - Incorporation of a piperazino-modified 2'-amino-LNA monomer (PipLNA-T) into oligonucleotides conferred very high affinity and base-pairing selectivity towards complementary DNA and RNA strands. Furthermore, one PipLNA-T modification provided a robust nuclease resistance that safeguarded three neighbouring natural nucleosides from 3'-exonucleolytic degradation. These favourable properties render PipLNA-T a promising oligonucleotide modification for various biological applications.

U2 - 10.1039/c5cc00322a

DO - 10.1039/c5cc00322a

M3 - Journal article

C2 - 25659978

VL - 51

SP - 4024

EP - 4027

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 19

ER -