NT-proBNP on Cobas h 232 in point-of-care testing: Performance in the primary health care versus in the hospital laboratory

Charlotte Gils, R. Ramanathan, T. Breindahl, M. Brokner, Anne Lindegaard Christiansen, Ø. Eng, I. J. Hammer, C. B. Herrera, A. Jansen, E. C. Langsjoen, E. S. Løkkebo, T. Osestad, A. D. Schroder, L. Walther

Research output: Contribution to journalJournal articleResearchpeer-review


Background. NT-proBNP may be useful for ruling out heart failure in primary health care. In this study we examined the analytical quality of NT-proBNP in primary health care on the Cobas h 232 point-of-care instrument compared with measurements performed in a hospital laboratory. Materials and methods. Blood samples requested for NT-proBNP were collected in primary health care (n = 95) and in a hospital laboratory (n = 107). NT-proBNP was measured on-site on Cobas h 232 instruments both in primary health care centres and at the hospital laboratory and all samples were also analyzed with a comparison method at the hospital. Precision, trueness, accuracy, and lot-variation were determined at different concentration levels and evaluated according to acceptance criteria. Furthermore user-friendliness was assessed by questionnaires. Results. For Cobas h 232 repeatability CV was 8.5-10.7% in the hospital setting and 5.3-10.0% in the primary health care and within the analytical quality specifications, but higher than with the comparison method (
Original languageEnglish
JournalScandinavian Journal of Clinical & Laboratory Investigation
Issue number7
Pages (from-to)602-609
Publication statusPublished - 2015


  • Humans
  • Laboratories, Hospital
  • Natriuretic Peptide, Brain/blood
  • Peptide Fragments/blood
  • Point-of-Care Testing/standards
  • Primary Health Care
  • Reproducibility of Results
  • Sensitivity and Specificity

Cite this

Gils, C., Ramanathan, R., Breindahl, T., Brokner, M., Lindegaard Christiansen, A., Eng, Ø., Hammer, I. J., Herrera, C. B., Jansen, A., Langsjoen, E. C., Løkkebo, E. S., Osestad, T., Schroder, A. D., & Walther, L. (2015). NT-proBNP on Cobas h 232 in point-of-care testing: Performance in the primary health care versus in the hospital laboratory. Scandinavian Journal of Clinical & Laboratory Investigation, 75(7), 602-609. https://doi.org/10.3109/00365513.2015.1066846